Tuyển chọn và định tên chủng nấm có khả năng sinh tổng hợp enzyme cellulase và xylanase=Screening and identifying mold strains capable of biosynthesizing enzyme cellulase and xylanase

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Tác giả: Thị Hồng Hà Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2024

Mô tả vật lý: tr.41-50

Bộ sưu tập: Báo, Tạp chí

ID: 475908

Thêm vào giỏ

Mục tiêu của nghiên cứu này là tuyển chọn chủng nấm được phân lập từ đất trồng dong riềng có khả năng phân giải cellulose và hemicellulose. Kết quả cho thấy, 36 chủng Trichoderma đã được phân lập và tuyển chọn được 3 chủng Trichoderma có khả năng phân giải cellulose và hemicellulose cao nhất là Trichoderma D5 có vòng phân giải cơ chất cellulose và xylan, tương ứng 3,02 cm và 3,43 cm
Trichoderma D19 là 2,65 cm và 2,88 cm
Trichoderma D25 là 2,86 cm và 2,61 cm. Cả 3 chủng Trichoderma đều có khả năng ức chế nấm gây bệnh Collectotrichum musae, Lasiodiplodia theobromae và Thielaviopsis paradoxa với tỷ lệ từ 74,1 - 91,3%
58,2 - 70,2% và 55,1 - 77,2%, theo thứ tự ở điều kiện in vitro. Ba chủng đã được định danh là: Trichoderma viride (T. viride D5), Trichoderma harzianum (T. harzianum D19), Trichoderma asperellum (T. asperellum D25) và được đăng kí trên GenBank với mã số tương ứng là: OR686312, OR690335, OR686311. Kết quả nghiên cứu cho thấy, tiềm năng phân giải cellulose và xylan của 3 chủng Trichoderma phân lập từ đất trồng dong riềng.The aim of this study was to select mold strains capable of degrading cellulose and hemicellulose. The results showed that among 36 Trichoderma strains, 3 Trichoderma strains were selected with the highest ability to degrade cellulose and hemicellulose: Trichoderma D5 had cellulose and xylan substrate degrading zone diameters of 3.02 cm and 3.43 cm
Trichoderma D19 got 2.65 cm and 2.88 cm
Trichoderma D25 got 2.86 cm and 2.61 cm, respectively. Three Trichoderma strains had strong antagonistic activity against pathogenic fungi of Colletotrichum musae, Lasiodiplodia theobromae and Thielaviopsis paradoxa from 74.1 - 91.3%, 58.2 - 70.2% and 55.1 - 77.2%, respectively under in vitro conditions. The three strains were identified as Trichoderma viride (T. viride D5), Trichoderma harzianum (T. harzianum D19) and Trichoderma asperellum (T. asperellum D25) and were registered on GenBank with the corresponding code OR686312
OR690335 and OR686311. These strains were considered to have the potential to decompose organic materials with high cellulose and hemicellulose content such as C. edulis Ker. residue.

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