Nhân dòng và giải trình tự một số gen tham gia vào quá trình tạo cơ và mỡ ở bò vàng Việt Nam=Cloning and sequencing of genes involved in muscle and fat formation in vietnam yellow cattle

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Tác giả: Văn Cường Đào

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2024

Mô tả vật lý: tr.62-70

Bộ sưu tập: Metadata

ID: 475911

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Muscle-derived satellite cells play a crucial role in muscle maintenance and regeneration. As a type of mesenchymal stem cell, they can differentiate into myogenic, fibrogenic, and adipogenic cells. Today, with Wagyu beef gaining popularity worldwide as well as in Vietnam, high-marbled beef is increasingly sought after. While Vietnam yellow cattle is raised for meat production, its carcass performance and marbling quality remain suboptimal for the increasingly demanding customers. To lay the foundation for in-depth research on the mechanisms of meat production and intramuscular fat formation in Vietnam yellow cattle, we employ Long-range PCR to clone and sequence genes involved in muscle development and intramuscular fat formation, namely, Pax3, Pax7, Mrf4, Myf5, Myod1, Myog and PPARƴ. This report presents the results of PCR cloning, Sanger sequencing and polymorphism detection of seven genes encoding for proteins involved in muscle development and intramuscular fat formation in the satellite cells derived from Vietnam yellow cattle.Tế bào gốc vệ tinh (satellite stem cell) đóng vai trò quan trọng trong quá trình duy trì và tái tạo cơ. Là một loại tế bào gốc thuộc nhóm trung mô, chúng có thể biệt hoá thành tế bào tiền thân của tế bào cơ, tiền thân tế bào sợi và tế bào mỡ. Ngày nay, với việc thịt bò Wagyu trở nên rất phổ biến trên thế giới cũng như tại Việt Nam, thịt bò có tỉ lệ mỡ giắt cao ngày càng được ưa chuộng. Tuy giống bò vàng Việt Nam được nuôi để lấy thịt, khả năng cho thịt cũng như tỉ lệ mỡ giắt của bò vàng còn chưa tối ưu. Nhằm đặt nền móng cho việc nghiên cứu sâu về cơ chế cho thịt cũng như tạo mỡ giắt ở giống bò vàng Việt Nam để cải thiện chất lượng thịt bò vàng thông qua lai giống, nghiên cứu ứng dụng kỹ thuật Long-Range PCR nhằm tạo dòng và giải trình tự các gen tham gia vào quá trình phát triển cơ và tạo mỡ giắt, gồm các gen Pax3, Pax7, Mrf4, Myf5,Myod1, MyMyog và PPARƴ. Bài viết này trình bày kết quả tạo dòng, giải trình tự bằng phương pháp Sanger và phân tích đa hình 7 gen mã hoá protein liên quan đến quá trình phát triển cơ cũng như kiểm soát tỉ lệ mỡ giắt của giống bò vàng Việt Nam, sử dụng nguyên liệu là tế bào gốc vệ tinh nuôi cấy in vitro .

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