Ảnh hưởng của liều lượng hormone GnRHa và DHA Selco bổ sung vào thức ăn đến kích thích quá trình thành thục sớm ở cá song da báo (Plectropomus leopardus Laepède, 1802)=Effects of implantation of different GnRH hormone dosages and dha selco supplementation on induction of sexual maturation of non-breeding season of grouper (plectropomus leopardus laepède, 1802)

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Tác giả: Anh Hiếu Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2024

Mô tả vật lý: tr.51-60

Bộ sưu tập: Metadata

ID: 475942

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Nghiên cứu này được thực hiện với 2 thí nghiệm nhằm mục đích đánh giá hiệu quả của việc áp dụng 2 phương pháp kích thích thúc đẩy quá trình thành thục sớm ở cá song da báo ( Plectropomus leopardus ) bố mẹ gồm cấy hormone GnRHa và bổ sung DHA Selco vào thức ăn ngay sau khi kết thúc mùa vụ sinh sản. Thí nghiệm 1 được bố trí với 4 nghiệm thức tương ứng với 3 liều lượng hormone GnRHa (25, 50, 70 µg/kg) và nghiệm thức đối chứng (không có hormone). Thí nghiệm 2 được bố trí với 1 nghiệm thức thí nghiệm (có bổ sung DHA Selco vào thức ăn) và một nghiệm thức đối chứng (không bổ sung DHA Selco). Các thí nghiệm được bố trí bằng phương pháp ngẫu nhiên hoàn toàn (CRD). Kết quả cho thấy, kích thích cá song da báo bằng phương pháp cấy hormone GnRHa đã thúc đẩy thời gian thành thục sớm hơn bình thường, với tỷ lệ thành thục >
40%. Kết quả cũng chỉ ra rằng, kích thích ở liều 25 hoặc 50 µg/kg đã giảm tỷ lệ dị hình so với ở liều 75 µg/kg. Ngược lại, tỷ lệ thành thục của cá bố mẹ ở lô đối chứng thấp (13,3%), nhưng không tham gia sinh sản. Ở thí nghiệm 2, bổ sung axit béo không no DHA vào thức ăn kết hợp kích thích bằng hormone GnRHa ở liều 50 µg/kg đã thúc đẩy thời gian thành thục sớm lên đến 70%, đạt tỷ lệ đẻ 87,5% và nâng cao tỷ lệ sống của ấu trùng 5 ngày tuổi.This study was conducted with two experiments to evaluate the effectiveness of applying two methods to promote early maturation of grouper Plectropomus leopardus, including GnRHa hormone implantation and DHA Selco supplementation in the feed of immature fish in the offspawning season. Experiment 1 was designed with 4 treatments of three doses of GnRHa hormone (25, 50, 70 μg/kg) and a control treatment. Experiment 2 was arranged with 1 experimental treatment (with DHA Selco supplementation in the feed) and 1 control treatment (without DHA Selco supplementation). Both experiments were carried out by using a completely randomized design (CRD). The results showed that stimulating grouper Plectropomus leopardus with GnRHa hormone implantation promoted maturation time earlier than normal, with a maturation rate >
40%. However, stimulation at doses of 25 or 50 μg/kg reduced the rate of larval deform compared with that at 75 μg/kg. Supplementation of the polyunsaturated fatty acid DHA in the feed combined with stimulation with the hormone GnRHa at a dose of 50 μg/kg promoted early maturation time by up to 70%, achieved a spawning rate of 87.5% and improved the survival rate of 5-day-old larvae.

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