Hoàn thiện quy trình công nghệ nhân giống hoa lan hoàng thảo bằng công nghệ nuôi cấy mô tế bào.=A study on the optimization of tissue culture parameters for the micropropagation of dendrobium sw. “den 08.5.2”

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Tác giả: Văn Tiến Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2024

Mô tả vật lý: tr.26-33

Bộ sưu tập: Metadata

ID: 475955

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Nghiên cứu nhằm hoàn thiện quy trình nhân giống in vitro lan hoàng thảo Den 08.5.2. đã được thực hiện. Kết quả nghiên cứu cho thấy: Vật liệu khởi đầu là chồi non in vivo được khử trùng bằng dung dịch nano bạc ở nồng độ 150 ppm trong 45 phút cho hiệu quả khử trùng tốt nhất và cao hơn công thức đối chứng với tỉ lệ mẫu sạch là 78,89%, tỉ lệ mẫu sạch và sống đạt 72,22%, thời gian phát sinh hình thái là 17,3 ngày. Môi trường nhân nhanh tối ưu là 2,5 g/L hyponex + 0,5 mg/L BAP + 0,2 mg/L α-NAA + 0,2 g/L than hoạt tính (THT) + 100 ml/L nước dừa đóng hộp (NDH) + 15 mg/L chitosan với hệ số nhân đạt 4,3 chồi/mẫu, chiều cao chồi 2,2 cm, khối lượng cụm chồi là 0,93 g/cụm, chồi xanh mập. Môi trường tạo cây hoàn chỉnh thích hợp là 2,5 g/L hyponex + 0,5 mg/L α-NAA + 0,3 g/L THT + 7,5 g/L bột chuối tiêu (BCT) với số rễ/cây đạt 5,38 rễ, chiều dài rễ là 4,17 cm, chiều cao cây 5,75 cm, số lá trên cây là 3,56 lá. Giai đoạn ra ngôi cây con lan hoàng thảo được ra ngôi trên giá thể dớn đạt hiệu quả tối ưu với tỷ lệ cây sống đạt 98,8% với chiều cao cây đạt 7,82 cm, khối lượng cây là 1,51 g/cây, tỷ lệ xuất vườn là 91,11%.This study aimed to develop an efficient in vitro propagation protocol for the Dendrobium orchid “Den 08.5.2”. Apical buds were effectively sterilized using a 150 ppm nano-silver solution for 45 minutes, resulting in a high rate of aseptic culture establishment (72.22%). Optimal shoot multiplication was achieved on a medium supplemented with 2.5 g/L Hyponex, 0.5 mg/L BAP, 0.2 mg/L α-NAA, 0.2 g/L activated charcoal (AC), 100 mL/L coconut juice and 15 mg/L chitosan. This medium yielded a multiplication rate of 4.3 shoots per explant, with an average shoot length of 2.2 cm and fresh weight of 0.93 g per shoot. Subsequent root development was optimized on a medium containing 2.5 g/L Hyponex, 0.5 mg/L α-NAA, 0.3 g/L AC and 7.5 g/L milled banana, resulting in 5.38 roots per plantlet, with an average root length of 4.17 cm, shoot length of 5.75 cm, and leaf number of 3.56 per plantlet. Finally, plantlets were successfully acclimatized to ex vitro conditions using Sphagnum moss as a substrate, demonstrating a high survival rate of 98.8% and achieving a plant height of 7.82 cm. This optimized protocol provides a foundation for the mass propagation of this valuable orchid cultivar

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