Phân tích hệ gen ty thể và mối quan hệ phát sinh chủng loại của gà trụi lông cổ=Analysis of the mitochondrial genome and phylogenetic relationships of naked neck chicken

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Tác giả: Thị Thanh Nhàn Giang, Thị Phương Mai Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Nông nghiệp và Phát triển nông thôn, 2024

Mô tả vật lý: tr.62-70

Bộ sưu tập: Metadata

ID: 475966

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In this study, the complete nucleotide sequence of the naked neck chicken mitochondrial genome, a Vietnamese indigenous chicken in Que Phong district, Nghe An province was determined by the Sanger sequencing method, analyzed structure and used to evaluate the phylogenetic relationship with different chicken breeds. The mitochondrial genome of naked neck chicken has a size of 16.785 bp, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 non-coding control region (D-loop). The proportion of A, T, G and C nucleotides were 30.28%, 23.75%, 13.48% and 32.48%, respectively. The mitochondrial genome sequence of the naked neck chicken was registered on GenBank with the accession number PQ412228. The analysis of phylogenetic relationships showed that the naked neck chicken originated from the red jungle fowl subspecies Gallus gallus spadiceus. The naked neck chicken was closely related to Vietnamese chicken breeds: Mong, Dong Tao and Cao Son dwarf chicken and Chinese chicken breeds: Rugao Yellow and Xiaoxang chicken.Trong nghiên cứu này, trình tự nucleotide hoàn chỉnh hệ gen ty thể của gà trụi lông cổ, một nguồn gen gà bản địa của Việt Nam thuộc huyện Quế Phong, tỉnh Nghệ An được xác định bằng phương pháp giải trình tự Sanger, phân tích cấu trúc và sử dụng để xác định mối quan hệ phát sinh chủng loại với các giống gà khác. Hệ gen ty thể của gà trụi lông cổ có kích thước là 16.785 cặp bazơ, bao gồm: 22 gen ARN vận chuyển (tRNA), 02 gen ARN ribosome (rRNA), 13 gen mã hóa protein và một vùng điều khiển không mã hóa (D-loop). Tỷ lệ các loại nucleotide thành phần A, T, G và C lần lượt tương ứng là 30,28%, 23,75%, 13,48% và 32,48%. Trình tự nucleotide toàn bộ hệ gen ty thể gà trụi lông cổ được đăng ký trên ngân hàng gen với mã số truy cập PQ412228. Kết quả phân tích quan hệ phát sinh chủng loại dựa trên trình tự hệ gen ty thể cho thấy, gà trụi lông cổ có nguồn gốc từ gà rừng lông đỏ phân loài Gallus gallus spadiceus. Gà trụi lông cổ có quan hệ gần gũi với gà Móng, gà Đông Tảo và gà lùn Cao Sơn của Việt Nam, gà vàng Rugao và gà Xiaoxang của Trung Quốc.

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