Phân tích ảnh hưởng của đột biến gen K13 của plasmodium falciparum đến nồng độ IC50 với một số thuốc điều trị sốt rét trên in vitro tại Gia Lai=In vitro evaluation of plasmodium falciparum K13 mutations on IC50 levels to some antimalarial drugs in Gia Lai

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Tác giả: Đình Cảnh Hoàng, Quang Thiều Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Y học cộng đồng, 2023

Mô tả vật lý: tr.155-161

Bộ sưu tập: Metadata

ID: 477713

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Phân tích ảnh hưởng của đột biến gen K13 của Plasmodium falciparum đến nồng độ IC50 với một số thuốc điều trị sốt rét trên In vitro tại Gia Lai, nhằm xác định tỷ lệ nhạy, kháng của thuốc với Plasmodium falciparum Đối tượng và phương pháp: Đề tài được thực hiện bằng phương pháp nghiên cứu mô tả thực nghiệm tại labo với 42 mẫu máu và mẫu ký sinh trùng nuôi cấy của bệnh nhân được chẩn đoán số rét do P. falciparum chưa biến chứng tại Gia Lai. Kết quả: IC50 trung bình của artesunat đối với Plasmodium falciparum là 3,06 ± 3,10 nmol/L
IC50 trung bình của dihydroartemisinin đối với Plasmodium falciparum 2,95 ± 2,19 nmol/L
IC50 trung bình của chloroquin đối với Plasmodium falciparum 67,7 ± 51,3 nmol/L
Nguy cơ kháng chloroquin ở các mẫu có gen K13 liên quan đến kháng thuốc cao gấp 1,47 lần mác mẫu không có gen K13 liên quan đến kháng thuốc ART, với OR = 1,47, 95%CI: 1,15-4,79
IC50 trung bình của piperaquin đối với Plasmodium falciparum 43,5 ± 24,9 nmol/L. Kết luận: IC50 trung bình của các mẫu có đột biến gen K13 cao hơn nhóm không có gen đột biến K13 đối với 4 loại thuốc ART gồm artesunat, dihydroartemisinin, chloroquin, piperaquin. Nguy cơ kháng chloroquin ở các mẫu có gen K13 liên quan đến kháng thuốc cao gấp 1,47 lần các mẫu không có gen K13 liên quan đến kháng thuốc ART.The study analyzes the influence of Plasmodium falciparum K13 mutations on IC50 levels to some anti-malarial drugs in vitro in Gia Lai to determine the drug sensitivity and resistance to Plasmodium falciparum. Subjects and methods: The study was conducted in the laboratory with a descriptive research design. Sume body included A total of 42 blood samples and cultured parasite samples from the patients diagnosed with uncomplicated P. falciparum malaria in Gia Lai were included. Results: The mean IC50 of artesunate for Plasmodium falciparum was 3.06 ± 3.10 nmol/L. The mean IC50 of dihydroartemisinin for Plasmodium falciparum was 2.95 ± 2.19 nmol/L. The mean IC50 of chloroquine for Plasmodium falciparum was 67.7 ± 51.3 nmol/L. The risk of chloroquine resistance in samples with drug resistance-related K13 genes was 1.47 times higher than that in samples without ART resistance-related K13 genes, with OR = 1.47, 95%CI: 1.15-4.79. The mean IC50 of piperaquine for Plasmodium falciparum was 43.5 ± 24.9 nmol/L. Conclusions: The mean IC50 of samples with K13 gene mutations was higher than that of those without K13 mutations for 4 ART drugs, including artesunate, dihydroartemisinin, chloroquine, and piperaquine. The risk of chloroquine resistance in samples with drug resistance-related K13 genes was 1.47 times higher than that in samples without ART resistance-related K13 genes.

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