Phân tích kiểu hình và kiểu gen của Klebsiella pneumoniae sản xuất carbapenemase tại thành phố Hồ Chí Minh, 2023=Phenotype and genotype analysis of Klebsiella pneumoniae producing carbapenemase in Ho Chi Minh city in 2023

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Tác giả: Minh Quỳnh Châu, Minh Tuấn Huỳnh, Tiến Dũng Nguyễn, Hùng Vân Phạm

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Y học Việt Nam (Tổng hội Y học Việt Nam), 2024

Mô tả vật lý: tr.46010

Bộ sưu tập: Metadata

ID: 478177

Thêm vào giỏ

Klebsiella pneumoniae (K. pneumoniae) bacteria cause bacterial infections with growing drug resistance, making treatment difficult. Objective: To determine the occurrence rate of carbapenemase-encoding genes and their phenotypic expression in K. pneumoniae strains isolated in Ho Chi Minh City in 2023. Methods: Agar diffusion and mCIM techniques were used to examine antibiotic resistance and carbapenemase secretion. Multiplex Real-time PCR was used to detect and determine the frequency of occurrence of carbapenemaseencoding genes. Results: A total of 230 K. pneumoniae strains were analyzed. Carbapenemase secretion was observed in 27% of strains. Third-generation cephalosporins resistance ranged from 78.7% to 100%. Carbapenem resistance was 27%, and other antibiotic groups had resistance rates between 15.8% and 52%. Among Carbapenem-resistant strains, the most frequent carbapenemase gene was OXA-48 (69.4%), followed by NDM-1 (64.5%) and KPC (4.8%). 30.4% of strains without Carbapenem resistance carried resistance genes. Conclusion: This study demonstrates the high level of drug resistance in K. pneumoniae, including resistance to third-generation Cephalosporins and Carbapenems. This poses a significant challenge in the treatment of K. pneumoniae infections. Strengthening drug resistance monitoring and developing effective treatments are essential.Vi khuẩn Klebsiella pneumoniae (K. pneumoniae) gây nên bệnh nhiễm khuẩn, có xu hướng gia tăng tính kháng thuốc gây khó khăn trong việc điều trị các bệnh do vi khuẩn này gây ra. Mục tiêu: Xác định tỷ lệ xuất hiện và biểu hiện kiểu hình, kiểu gen mã hoá carbapenemase ở các chủng K. pneumoniae phân lập được tại Thành phố Hồ Chí Minh trong năm 2023. Phương pháp: Kỹ thuật kháng sinh đồ khuếch tán trên thạch và mCIM để khảo sát đề kháng kháng sinh và tiết carbapenemase. Kỹ thuật Multiplex Real-time PCR (MPL-rPCR) với độ nhạy, độ đặc hiệu cao giúp phát hiện và xác định tần suất xuất hiện của các gen mã hoá carbapenemase. Kết quả: Trong 230 chủng K. pneumoniae, tỷ lệ K. pneumoniae tiết carbapenemase là 27%. Cephalosporin thế hệ 3 kháng từ 78,7% - 100%. Kháng Carbapenem 27% và các nhóm kháng sinh khác 15,8 - 52%. Tần suất xuất hiện gen mã hoá ở các chủng kháng Carbapenem cao nhất là OXA-48 chiếm 69,4%, tiếp đến NDM-1 64,5% và KPC là 4,8%. Tỷ lệ chủng không biểu hiện kháng Carbapenem nhưng mang gen kháng chiếm 30,4%. Kết luận: Nghiên cứu này cho thấy tình trạng kháng thuốc cao của K. pneumoniae, bao gồm cả kháng thuốc Cephalosporin thế hệ 3 và Carbapenem. Điều này gây ra thách thức lớn trong điều trị nhiễm khuẩn do K. pneumoniae và tăng cường theo dõi xu hướng kháng thuốc và phát triển các phương pháp điều trị hiệu quả là rất cần thiết.

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