Đánh giá hiệu quả của phương pháp thử nghiệm độ nhạy cảm kháng sinh trực tiếp từ môi trường cấy máu dương tính tại Bệnh viện Đa khoa Thống Nhất tỉnh Đồng Nai=Evaluation of the effectiveness of direct antimicrobial susceptibility testing from positively flagged blood culture bottles at Thong Nhat General Hospital of Dong Nai Province

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Tác giả: Trần Linh Đan Lưu, Sĩ Tuấn Nguyễn, Thị Huệ Vân Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Y học Việt Nam (Tổng hội Y học Việt Nam), 2024

Mô tả vật lý: tr.149-156

Bộ sưu tập: Metadata

ID: 478205

Thêm vào giỏ

Early identification of patients with sepsis helps clinicians choose appropriate antibiotics and respond promptly, bringing high treatment effectiveness and reducing costs for patients. The goal of this research is to find a new method to diagnose sepsis quickly and give highly accurate results. Research method: blood samples were cultured into blood culture bottles with positive results for bacteria, then direct antimicrobial susceptibility testing (AST) from the positively flagged blood culture bottles according to CLSI and EUCAST guidelines. Results: Among 82 samples positive for bacteria, samples were contaminated with E. coli (45.2%), S.aureus (29.3%), K. pneumoniae (12.2%), A. baumannii (7.3%), P. mirabilis (2.4%), Salmonella sp. (2.4%), and P. aeruginosa (1.2%). The direct AST method after 16 hours of incubation produced the best results, with CA reaching 93.2%, VME 0.4%, ME 4.8%, mE 4.0%, and ATU reduced to 2. The time to get direct AST results compared to the standard method decreased by an average of 29.35 hours (more than 1 day), with p<
0.0001. Conclusion: The direct AST method gives faster results than the routine method.Tìm một phương pháp mới để chẩn đoán NKH nhanh và cho kết quả chính xác cao. Phương pháp nghiên cứu: mẫu máu được cấy vào chai cấy máu cho kết quả dương tính với vi khuẩn, sau đó tiến hành thử nghiệm độ nhạy cảm kháng sinh (AST) trực tiếp từ môi trường cấy máu dương tính theo hướng dẫn của CLSI và EUCAST. Kết quả: Trong 82 mẫu dương tính với vi khuẩn, mẫu nhiễm E. coli (45,2%), S. aureus (29,3%), K. pneumoniae (12,2%) A. baumannii (7,3%), P. mirabilis (2,4%), Salmonella sp. (2,4%) và P. aeruginosa (1,2%). Phương pháp AST trực tiếp tại mốc đọc sau 16 giờ ủ cho kết quả tốt nhất với CA đạt 93,2%, VME là 0,4%, ME là 4,8% và mE là 4,0% và ATU giảm còn 2. Thời gian có kết quả AST trực tiếp so với phương pháp tiêu chuẩn giảm trung bình 29,35 giờ (hơn 1 ngày), với p<
0,0001. Kết luận: Phương pháp AST trực tiếp cho kết quả nhanh hơn so với phương pháp tiêu chuẩn.

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