Nghiên cứu điều chế vi cầu chứa bovine serum albumin từ alginat và chitosan=Research on the preparation of microspheres containing bovine serum albumin microspheres from alginate and chitosan

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Tác giả: Minh Quân Lê

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học và Công nghệ Việt Nam, 2024

Mô tả vật lý: tr.29-36

Bộ sưu tập: Metadata

ID: 478392

Thêm vào giỏ

Microspheres are controlled-release drug delivery systems with a micron diameter with a polymeric structure. Alginate and chitosan are two pharmaceutical biodegradable polymers applied for various microsphere preparation techniques. However, there is very limited research on microspheres from alginate and chitosan in Vietnam. Thus, the goal of this research was to prepare and optimise the alginate microspheres containing the model protein bovine serum albumin (BSA) preparation process and then develop a chitosan coating method. The developed method of alginate microspheres containing BSA includes alginate, BSA, isooctane, span 80, tween 80, and calcium chloride. Formulations and process parameters influencing the alginate microsphere properties were optimised using Design Expert v12.0 software. Alginate microspheres containing BSA were coated with 1% chitosan in 1% acetic acid and achieved the controlled release of BSA from microspheres in 24 hours. Chitosan-coated alginate microspheres containing BSA had an average particle diameter of 178 μm, in which the drug loading rate was 12.7%. This study successfully prepared the controlled-release microspheres containing BSA from alginate and chitosan polymers.Vi cầu là hệ thống phân phối có khả năng kiểm soát giải phóng dược chất kích thước micron, có polymer trong cấu trúc. Trong đó, alginat và chitosan là hai polymer tương thích sinh học có ứng dụng điều chế vi cầu đa dạng trong dược phẩm. Tuy nhiên, ở Việt Nam hiện vẫn chưa có nhiều công bố về vi cầu từ alginat và chitosan. Vì vậy, nghiên cứu được tiến hành để xây dựng và tối ưu hóa quy trình điều chế vi cầu lõi alginat chứa protein mô hình bovine serum albumin (BSA), sau đó xây dựng phương pháp bao phủ chitosan. Quy trình điều chế vi cầu lõi được xây dựng gồm alginat, BSA, isooctan, span 80, tween 80 và calci clorid. Các yếu tố công thức và thông số quy trình được khảo sát bằng phần mềm Design Expert v12.0. Vi cầu lõi alginat chứa BSA được bao phủ bằng 1% chitosan trong acid acetic 1% và có khả năng giải phóng kiểm soát BSA trong 24 giờ. Vi cầu alginat bao phủ chitosan có kích thước trung bình 178 μm, tỷ lệ tải BSA đạt 12,7%. Nghiên cứu đã điều chế thành công vi cầu chứa BSA từ hai polymer alginat và chitosan có khả năng giải phóng dược chất có kiểm soát.

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