Đánh giá biểu hiện của phân tử microRNA osa-miR160a trên các giống lúa chống chịu và mẫn cảm với nấm Magnaporthe oryzae gây bệnh đạo ôn=Expression profile of microRNA osa-miR160a in Magnaporthe oryzae resistant and susceptible rice cultivars

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Tác giả: Bằng Phi Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học và Công nghệ Việt Nam, 2025

Mô tả vật lý: tr.58-61

Bộ sưu tập: Metadata

ID: 478433

Thêm vào giỏ

Bệnh đạo ôn ở lúa do nấm Magnaporthe oryzae gây ra làm thiệt hại năng suất lúa mỗi năm đủ để nuôi sống 60 triệu người. Những nghiên cứu gần đây cho thấy, các phân tử microRNAs liên quan đến khả năng chống chịu với nấm M. oryzae. Trong đó, osa-miR160 được báo cáo là có vai trò điều hòa tăng hiệu quả chống chịu nấm M. oryzae bằng cách kiểm soát các gen đáp ứng với auxin, ARFs (auxin response transcription factors) trên cây lúa. Trong nghiên cứu này, các tác giả phân tích và đánh giá mức độ tích lũy của osa-miR160a trên các giống lúa mẫn cảm và chống chịu với bệnh đạo ôn được trồng tại Việt Nam dựa trên kỹ thuật qRT-PCR. Mặc dù mức độ biểu hiện của osa-miR160a có sự thay đổi khác nhau giữa các giống lúa tại các thời điểm sau khi nhiễm (hpi), nhưng chỉ tại thời điểm 24 hpi mới có sự khác biệt có ý nghĩa thống kê về biểu hiện của osa-miR160a giữa nhóm lúa kháng và nhóm lúa mẫn cảm với bệnh đạo ôn. Mức độ biểu hiện của osa-miR160a ở thời điểm 24 hpi trên nhóm lúa kháng cao gấp 2,6 lần so với trên nhóm lúa mẫn cảm. Kết quả này cho thấy, osa-miR160a là một chỉ thị phân tử tiềm năng trong việc đánh giá khả năng chống chịu với nấm M. oryzae của các giống lúa trồng tại Việt Nam hiện nay.Rice blast is caused by the fungus Magnaporthe oryzae due to rice blast enough to feed 60 million people. Recent studies have shown that microRNAs are involved in the rice immunity against M. oryzae. In which, osa-miR160 was reported to play a role in upregulating resistance to M. oryzae through the inhibition of auxin response transcription factors (ARFs) genes. In this study, we analysed and evaluated the expression level of osa-miR160a on blast-resistant and susceptible rice groups grown in Vietnam by the qRT-PCR method. Although the expression of osa-miR160a showed different levels of change in rice cultivars at different hours post-inoculation (hpi), only at 24 hpi was there a statistically significant difference in the osa-miR160a expression between blast-resistant and susceptible rice groups. The expression level of osa-miR160a at 24 post-inoculation (hpi) in the blast-resistant rice group was 2.6 times higher than in the susceptible group. The result showed that osa-miR160a would be a potential molecular marker in assessing the resistance against M. oryzae of different rice cultivars grown in Vietnam.

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