Nghiên cứu vi bao kháng thể IgY=Research on microcapsules for IgY antibodies

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Tác giả: Thành Đạt Huỳnh

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Khoa học và Công nghệ Việt Nam, 2025

Mô tả vật lý: tr.75-80

Bộ sưu tập: Metadata

ID: 478440

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IgY antibodies have been shown to be effective in the prevention and treatment of some infectious diseases, such as influenza A and hand, foot and mouth disease (HFMD)... Chitosan-alginate microcapsules are considered an effective approach to delivering IgY into the body via the oral route. The research on IgY microcapsules for IgY antibodies investigated the effects of encapsulating agents and encapsulation conditions on the efficiency of the microcapsules process of IgY antibodies against Enterovirus 71 (EV71), including: alginate concentration, chitosan concentration, microcapsules environment pH. The results determined the optimal parameters to enhance the efficiency of microcapsules IgY, which are 2.5% (w/v) sodium alginate, 0.2% chitosan, and microcapsules environment pH 3,5. Under optimum conditions, IgY microcapsules efficiency was 63.93%. After freeze-dried microcapsules, the activity against EV71 in the microcapsules was 35.01%. The IgY stability in simulated gastric fluid (SGF) was significantly improved when encapsulated by chitosan-alginate microcapsules, and the activity of IgY was fully maintained for 3 hours in SGF with the appearance of pepsin. Scanning electron microscopy (SEM), results showed that the microcapsule particle size is ≤10 μm, the microcapsule particle structure is porous, and there are many microcapsule particles in the microcapsule product after freeze-drying.Kháng thể IgY đã được chứng minh có hiệu quả phòng chống và điều trị một số bệnh truyền nhiễm như cúm A, bệnh tay chân miệng… Vi bao chitosan-alginate được đánh giá là một phương pháp hiệu quả để đưa kháng thể IgY vào cơ thể qua đường ăn uống. Nghiên cứu vi bao kháng thể IgY đã tiến hành khảo sát ảnh hưởng của các chất bao và điều kiện vi bao đến hiệu quả của quá trình vi bao kháng thể IgY kháng Enterovirus 71 (EV71) bao gồm: nồng độ sodium alginate, nồng độ chitosan, pH môi trường vi bao. Kết quả thí nghiệm đã đưa ra các thông số tối ưu nhằm tăng hiệu quả vi bao kháng thể IgY như sau: nồng độ sodium alginate 2,5% (w/v), nồng độ chitosan 0,2%, pH môi trường vi bao 3,5. Trong điều kiện tối ưu, hiệu quả vi bao đạt 63,93%. Chế phẩm vi bao sau sấy có hoạt tính kháng thể IgY kháng EV71 còn lại trong hạt vi bao đạt 35,01%. Độ ổn định của IgY trong môi trường mô phỏng dạ dày (SGF) đã được cải thiện đáng kể, khi được vi bao bằng chitosan-alginate, hoạt tính kháng thể của IgY được giữ lại đến 3 giờ trong SGF có pepsin. Kết quả chụp SEM cho thấy, kích thước hạt vi bao ≤10 μm, cấu trúc hạt vi bao xốp, có rất nhiều hạt vi bao trong một hạt chế phẩm vi bao sau sấy tạo thành khối hạt vi bao.

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