KHẢO SÁT HOẠT TÍNH KHÁNG OXY HÓA VÀ KHÁNG VIÊM IN VITRO CỦA VI KHUẨN NỘI SINH TRONG CÂY DỪA CẠN (Catharanthus roseus)=INVESTIGATION OF IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ENDOPHYTIC BACTERIA IN Catharanthus roseus

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Tác giả: Văn Trương Huỳnh, Khôi Nguyên Lý, Tấn Thành Nguyễn, Thị Kim Ngân Nguyễn, Lâm Tài Tạ, Chí Linh Trần, Nguyễn Kim Ngân Trần, Hoàng Long Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Thái Nguyên, 2024

Mô tả vật lý: tr.254 - 261

Bộ sưu tập: Báo, Tạp chí

ID: 480411

Thêm vào giỏ

Nghiên cứu được thực hiện nhằm tuyển chọn các dòng vi khuẩn nội sinh trong cây dừa cạn có khả năng kháng oxy hoá và kháng viêm in vitro. Nghiên cứu đã phân lập và nhận diện sơ bộ các đặc điểm hình thái của 11 dòng vi khuẩn nội sinh trong cây dừa cạn. Hoạt tính kháng oxy hóa của các dòng vi khuẩn nội sinh được xác định nhờ vào phương pháp kháng oxy hóa tổng số, năng lực khử và trung hòa gốc tự do 2,2-diphenyl-1-picrylhydrazyl. Hoạt tính kháng viêm của các dòng vi khuẩn nội sinh được xác định dựa vào khả năng ức chế sự biến tính albumin huyết thanh bò. Các dòng vi khuẩn nội sinh có khả năng sản sinh chất kháng oxy hóa với hàm lượng dao động từ 5,34±0,26 đến 175,44±0,46 mg AAE/mL. Hàm lượng chất kháng viêm của các dòng vi khuẩn nội sinh dao động từ 14,35±0,54 đến 30,35±0,20 mg DE/mL. Kết quả nghiên cứu cho thấy, hoạt tính kháng oxy hóa và kháng viêm của các dòng vi khuẩn nội sinh phụ thuộc vào hàm lượng polyphenol và flavonoid. Các dòng vi khuẩn nội sinh trong cây dừa cạn cho thấy tiềm năng sản sinh các các hợp chất chuyển hóa thứ cấp có khả năng kháng oxy hóa và kháng viêm.The study was conducted to select endophytic bacterial strains in Catharanthus roseus that have anti-oxidant and anti-inflammatory properties in vitro. The study isolated and preliminary identified the morphological characteristics of 11 strains of endophytic bacteria in Catharanthus roseus. Antioxidant activity of endophytic bacterial strains was determined by the method of total antioxidant capacity, reducing power and neutralizing 2,2-diphenyl-1-picrylhydrazyl free radical. The anti-inflammatory activity of endophytic bacterial strains was determined based on their ability to inhibit bovine serum albumin denaturation. Endophytic bacterial strains are capable of producing antioxidants with concentrations ranging from 5.34±0.26 to 175.44±0.46 mg AAE/mL. The anti-inflammatory content of endophytic bacterial strains ranges from 14.35±0.54 to 30.35±0.20 mg DE/mL. Research results show that the antioxidant and anti-inflammatory activities of endophytic bacterial strains depend on polyphenol and flavonoid content. Endophytic bacterial strains in Catharanthus roseus show the potential to produce secondary metabolites with antioxidant and anti-inflammatory properties.

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