Reproductive Tract Infections (RTI) are common in Vietnam. RTI knowledge of healthcare staff at grassroot health care service is very important for prevention and control their complications. The study was carried out to describe RTI knowledge among health staff in Ba Vi district, Hanoi. The results show that, most of health staff responded correctly about few common diseases, symptoms, causes and risk factors, or complications of RTI. The number of health staff participated any RTI training course was low. In conclusions, comprehensive knowledge about RTI of the health staff in Ba Vi district was limited. Intervention activities to improve knowledge on RTI for heath staff at grassroot healthcare service are very important and needed for early diagnosis and tre?tment of RTI.