This study aims to analyze factors influencing construction enterprises’ business efficiency in the Mekong Delta region. The research data comprises 100 enterprises operating in provinces such as Bac Lieu, Ca Mau, and Can Tho City. The multiple regression method is used to test the correlation between factors. Estimated results show that factors influencing the business efficiency of construction enterprises in the Mekong Delta region include the educational level of business owners, management experience, and type of enterprise. Among these, the management experience of business owners is the strongest factor impacting the business efficiency of construction enterprises in the Mekong Delta region.