Xác định hàm lượng một số chất nhóm NSAIDs trong thịt bằng sắc ký lỏng ghép nối khối phổ hai lần (LC-MS/MS)=Determination of some NSAIDs content in meat by liquid chromatography with tandem mass spectrometry (LC-MS/MS)

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2024

Mô tả vật lý: tr.168-181

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ID: 481728

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Nonsteroidal anti-inflammatory drugs (NSAIDs) are the most widely used group of drugs worldwide for pain relief, anti-inflammation, blood clot prevention, and fever reduction, but they can cause some side effects bad for health if used incorrectly. NSAIDs are on the list of drugs that must be monitored according to Council Directive 96/23/EC. The fact that NSAIDs are also used in animals for anti-inflammation, fever reduction, and pain relief raises concerns that animal products such as meat, eggs, and milk may be contaminated with NSAIDs, affecting human health if consumed like eating right. Therefore, developing a method to analyze NSAIDs in meat products is necessary, contributing to assessing the quality of meat products currently circulating on the market. In this study, 4 common substances in the NSAIDs group were studied and identified by liquid chromatography with tandem mass spectrometry (LC-MS/MS) using a Symmetry C18 chromatographic column with an ESI (+) positive electron spray ionization source and multiple reaction monitoring (MRM) mode. The validity of the method was confirmed according to the guidelines of the Association of Official Analytical Chemists (AOAC). The results showed that the method has good specificity, the linearity range from 5.0 to 100 µg/kg, the detection limit of 1.5 µg/kg, the quantification limit of 5.0 µg/kg
precision and accuracy with relative standard deviation (RSD) less than 11% and recoveries ranging from 81.1 to 111%, meeting AOAC requirements. The method was used to analyze the content of NSAIDs in 15 meat samples in Hanoi. The results showed that 7 samples showed tolfenamic acid exceeding the quantitative limit of the method.Thuốc giảm đau, chống viêm không steroid (NSAIDs) là nhóm thuốc được sử dụng rộng rãi nhất trên toàn thế giới để giảm đau, chống viêm, ngăn ngừa cục máu đông và hạ sốt, nhưng chúng có thể gây ra một số tác động xấu đến sức khỏe nếu sử dụng sai cách. NSAIDs nằm trong danh mục các loại thuốc phải được giám sát theo Hội đồng Chỉ thị 96/23/EC. Việc NSAIDs còn được dùng cho động vật để chống viêm, hạ sốt và giảm đau làm dấy lên lo ngại về các sản phẩm từ động vật như thịt, trứng, sữa có thể bị nhiễm NSAIDs, gây ảnh hưởng đến sức khỏe con người nếu như ăn phải. Vì vậy, việc xây dựng một phương pháp để phân tích NSAIDs trong các sản phẩm thịt là cần thiết, góp phần đánh giá chất lượng các sản phẩm thịt đang được lưu hành trên thị trường. Trong nghiên cứu này, 4 chất phổ biến trong nhóm NSAIDs đã được nghiên cứu xác định bằng sắc ký lỏng khối phổ (LC-MS/MS) sử dụng cột sắc ký Symmetry C18, nguồn ion hóa phun điện tử ion dương ESI (+), chế độ theo dõi ion đa phản ứng (MRM - Multiple reaction monitoring). Giá trị sử dụng của phương pháp được xác nhận theo hướng dẫn của Hiệp hội các nhà hóa học phân tích chính thức (AOAC). Kết quả cho thấy phương pháp có tính đặc hiệu tốt, đường chuẩn được xây dựng trong khoảng nồng độ từ 5,0 – 100 µg/kg, giới hạn phát hiện là 1,5 µg/kg, giới hạn định lượng là 5,0 µg/kg
độ chụm và độ đúng với độ lệch chuẩn tương đối (RSD) nhỏ hơn 11% và độ thu hồi dao động từ 81,1 – 111%, đáp ứng yêu cầu theo AOAC. Phương pháp đã được sử dụng để phân tích hàm lượng NSAIDs trong 15 mẫu thịt trên địa bàn Hà Nội. Kết quả cho thấy có 7 mẫu xuất hiện tolfenamic acid vượt qua giới hạn định lượng của phương pháp.

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