Phân lập, tuyển chọn và định danh vi khuẩn lactic từ củ sen muối chua tại huyện Giang Thành, tỉnh Kiên Giang=Isolation, selection and identification of lactic bacteria from pickled salt lotus root in Giang Thanh district, Kien Giang province

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Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2024

Mô tả vật lý: tr.389-399

Bộ sưu tập: Metadata

ID: 481813

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Nghiên cứu được thực hiện nhằm mục đích phân lập, tuyển chọn và định danh được dòng vi khuẩn lactic có khả năng lên men lactic cao nhất từ sản phẩm củ sen muối chua tại huyện Giang Thành, tỉnh Kiên Giang. Từ ba mẫu củ sen muối chua thu thập tại huyện Giang Thành, 11 chủng vi khuẩn lactic đã được phân lập và nhận diện sơ bộ thông qua hình thái khuẩn lạc và một số phản ứng sinh hoá như nhuộm Gram, phản ứng catalase. Kết quả xác định kiểu lên men cho thấy cả 11 chủng vi khuẩn phân lập được đều có kiểu lên men đồng hình, sản phẩm lên men chủ yếu là acid lactic. Trong đó, dòng D15 có hàm lượng acid lactic sinh ra cao nhất, đạt 1,87 mg/mL và có khả năng phát triển sinh khối cao nhất, giá trị OD600nm đạt 0,947. Đã xác định được trình tự gen 16S rRNA của dòng D15 có kích thước phân tử 1469 bp. So sánh trình tự gen 16S rRNA của dòng D15 trên ngân hàng gen có mức độ tương đồng 100% với một số chủng vi khuẩn lactic thuộc loài Lactobacillus plantarum và 99,68% khi so sánh cặp với trình tự gen 16S rRNA của chủng chuẩn loài Lactobacillus plantarum. Tiến hành xây dựng cây phát sinh loài và đánh giá quan hệ di truyền của dòng D15, chủng chuẩn loài Lactobacillus plantarum và 9 chủng có độ tương đồng cao nhất với dòng D15 dựa trên trình tự gen 16S rRNA. Kết quả phân loại thành 2 nhóm lớn có độ tương đồng 92%, trong đó dòng D15 cùng nhóm với chủng chuẩn loài Lactobacillus plantarum và cũng có độ tương đồng 92%.The study was conducted to isolate, select, and identify the lactic acid bacteria strain with the highest lactic acid fermentation ability from pickled lotus root products in Giang Thanh district, Kien Giang province. From three samples of pickled lotus root collected in Giang Thanh district, 11 lactic acid bacteria strains were isolated and preliminarily identified through colony morphology and some biochemical reactions such as Gram staining and catalase reaction. The results of determining the fermentation type showed that all 11 isolated bacterial strains had a homomorphic fermentation type, the main fermentation product was lactic acid. Of which, strain D15 had the highest lactic acid content, reaching 1.87 mg/mL and had the highest biomass growth ability, with an OD600nm value of 0.947. The 16S rRNA gene sequence of strain D15 was determined with a molecular size of 1469 bp. Comparing the 16S rRNA gene sequence of strain D15 on the gene bank, it has a similarity level of 100% with some lactic acid bacteria strains of the species Lactobacillus plantarum and 99.68% when comparing with the 16S rRNA gene sequence of the standard strain Lactobacillus plantarum. Constructing a phylogenetic tree and evaluating the genetic relationship of strain D15, the standard strain Lactobacillus plantarum, and 9 strains with the highest similarity to strain D15 based on the 16S rRNA gene sequence. The results were classified into 2 large groups with a similarity level of 92%, in which strain D15 is in the same group as the standard strain Lactobacillus plantarum and also has a similarity level of 92%.

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