Nghiên cứu vi khuẩn Pseudomonas spp. có nguồn gốc từ thịt lợn và thịt gà=Study on Pseudomonas spp. isolated from pork and chicken meat

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Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2024

Mô tả vật lý: tr.420-428

Bộ sưu tập: Metadata

ID: 481819

Thêm vào giỏ

Pseudomonas spp. are heterotrophic, Gram-negative, aerobic bacteria widely distributed in the environment, acting as food spoilage agents and opportunistic pathogens in humans. In this study, we applied the ISO 13720:2010 for enumeration of presumptive Pseudomonas spp. in meat and meat products to investigate the contamination of Pseudomonas spp. in pork and chicken meat samples collected from markets in various districts of Ho Chi Minh City. The results indicated that 78% of pork samples and 72% of chicken samples were contaminated with Pseudomonas spp., with average densities of 4.9×106 CFU/g and 6.8×106 CFU/g, respectively. Almost isolated Pseudomonas strains could grow at neutral and alkaline pH, with an optimal temperature range of 10°C to 44°C, and tolerate to NaCl 5%. These findings provide critical information on the distribution and growth characteristics of Pseudomonas spp. in meat, therefore, being a premise to propose the research on density thresholds to ensure food quality.Pseudomonas spp. là vi khuẩn dị dưỡng, gram âm, hiếu khí, phân bố rộng rãi trong môi trường và là tác nhân gây thực phẩm cũng như bệnh cơ hội ở người. Trong nghiên cứu này, chúng tôi đã sử dụng quy trình định lượng Pseudomonas spp. giả định trong thịt và các sản phẩm thịt theo tiêu chuẩn ISO 13720:2010 để nghiên cứu sự ô nhiễm vi khuẩn Pseudomonas spp. trong mẫu thịt lợn và thịt gà thu thập tại các chợ ở các quận/huyện thuộc khu vực thành phố Hồ Chí Minh. Kết quả cho thấy 78% mẫu thịt lợn và 72% mẫu thịt gà nhiễm Pseudomonas spp., với mật độ trung bình lần lượt là 4.9x106 CFU/g và 6.8x106 CFU/g. Đa số các chủng Pseudomonas spp. phân lập có khả năng phát triển ở pH trung tính và kiềm, với nhiệt độ 10°C đến 44°C và chịu được nồng độ muối NaCl lên đến 5%. Những kết quả này cung cấp thông tin quan trọng về sự phân bố và đặc điểm sinh trưởng của Pseudomonas spp. có nguồn gốc từ thịt, từ đó, làm tiền đề để đề xuất những nghiên cứu về ngưỡng mật độ đảm bảo chất lượng thực phẩm.

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