Xác định đồng thời acid carnosic và carnosol trong thực phẩm chứa lá hương thảo bằng phương pháp sắc ký lỏng hiệu năng cao (HPLC–PDA)=Simultaneous determination of acid carnosic and carnosol in rosemary-containing foods by high-performance liquid chromatography with photodiode array detection (HPLC

Xác định đồng thời acid carnosic và carnosol trong thực phẩm chứa lá hương thảo bằng phương pháp sắc ký lỏng hiệu năng cao (HPLC–PDA)=Simultaneous determination of acid carnosic and carnosol in rosemary-containing foods by high-performance liquid chromatography with photodiode array detection (HPLC–PDA)

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả:

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Kiểm nghiệm và An toàn thực phẩm, 2024

Mô tả vật lý: tr.481-493

Bộ sưu tập: Báo, Tạp chí

ID: 481832

Thêm vào giỏ

Nghiên cứu được thực hiện với mục tiêu xây dựng và thẩm định phương pháp định lượng đồng thời acid carnosic và carnosol trong thực phẩm chứa lá hương thảo bằng kỹ thuật sắc ký lỏng hiệu năng cao kết hợp detector chuỗi diod quang (HPLC-PDA), với quy trình xử lý mẫu đơn giản, nhanh chóng. Mẫu phân tích được chiết bằng kỹ thuật lắc ngang với hỗn hợp methanol - acid phosphoric (99,5:0,5, v/v) ở nhiệt độ phòng trong 20 phút. Các điều kiện sắc ký như sau: cột Sunfire C18 (4,6 mm x 250 mm
5 µm), pha động gồm acid phosphoric 0,1% – acetonitrile (40:60, v/v), tốc độ dòng 1 mL/phút, thể tích tiêm 10 µL, phát hiện ở bước sóng 230 nm. Phương pháp có độ đặc hiệu tốt, đường chuẩn của acid carnosic và carnosol tuyến tính trong khoảng nồng độ 2,5 – 200 µg/mL. Giới hạn phát hiện (LOD) của acid carnosic và carnosol lần lượt là 0,3 µg/mL và 0,25 µg/mL. Giới hạn định lượng (LOQ) của acid carnosic và carnosol lần lượt là 1 µg/mL và 0,75 µg/mL. Độ thu hồi trong khoảng 98,8 – 100,6% (acid carnosic) và 97,5 – 102,2% (carnosol). Độ lặp lại và độ tái lặp nội bộ đạt yêu cầu theo AOAC. Phương pháp đã được áp dụng để đánh giá hàm lượng acid carnosic và carnosol trong 20 mẫu thực phẩm thu thập trên thị trường như bột lá hương thảo, lá hương thảo khô, trà thảo mộc hương thảo,...The research was conducted to develop and validate a simultaneous quantification method for acid carnosic (CA) and carnosol (CAR) in rosemary-containing foods using highperformance liquid chromatography coupled with photodiode array detection (HPLC–PDA), with a simple and fast sample processing procedure. Analytical samples were extracted using a methanol–phosphoric acid mixture (99.5:0.5, v/v) at room temperature for 20 minutes. The chromatographic conditions were as follows: Sunfire C18 column (4.6 mm x 250 mm
5 µm), mobile phase consisting of 0.1% phosphoric acid – acetonitrile (40:60, v/v), flow rate of 1 mL/min, injection volume of 10 µL, detection at 230 nm. The method was validated according to the Association of Official Analytical Chemists (AOAC) criteria. The results showed that the method had good specificity, and the standard curves of acid carnosic and carnosol were linear in the concentration range of 2.5 – 200 µg/mL. The limits of detection (LOD) for acid carnosic and carnosol were 0.3 µg/mL and 0.25 µg/mL, respectively. The limits of quantification (LOQ) for acid carnosic and carnosol were 1 µg/mL and 0.75 µg/mL, respectively. The recovery rates were in the range of 98.8 – 100.6% (acid carnosic) and 97.5 – 102.2% (carnosol). Repeatability and internal reproducibility met the requirements of AOAC. The method was applied to evaluate the acid carnosic and carnosol levels in 20 food samples collected from the market, such as rosemary leaf powder, dried rosemary leaves, rosemary herbal tea,...

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR