Nghiên cứu xác định một số yếu tố liên quan đến quá trình tạo cây khoai tây nhị bội (2n=4x) từ cây tứ bội solanum tuberosum (2n=4x) theo hướng trinh sinh / Nghiên cứu xác định mức bổ sung và ảnh hưởng của bentonite trong thức ăn nuôi gà dò hậu bị và đẻ trứng / Nghiên cứu xác định nấm Phomopsis durionis và Lasiodiplodia theobromae gây bệnh cháy lá trên sầu riêng

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Tác giả: Thị Kim Uyên Đặng, Thị Thu Hà Đỗ, Lam Sơn Hồ, Thị Giang Hoàng, Thị Tưởng Lê, Thị Hồng Lương, Quang Thạch Nguyễn, Thị Phương Thảo Nguyễn, Văn Hòa Nguyễn, Công Thiếu Phạm, Thị Hằng Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 631636635 Specific techniques; apparatus, equipment, materials

Thông tin xuất bản: Tạp chí nông nghiệp và phát triển nông thôn, Khoa học công nghệ chăn nuôi, Tạp chí Khoa học và Công nghệ Nông nghiệp Việt Nam, 201220072022

Mô tả vật lý: 18-23, 23-27, 57 - 60

Bộ sưu tập: Metadata

ID: 481905

Thêm vào giỏ

The research was to detennine the method for producing diploid (2n=2x) potato plants through parthenogenesis by crossing the tetraploid Solanum tuberosum (2n=4x) and the wild-type potato Solanum phureja. The materials which were used in this research, the S. tuberosum cultivars Fasan, Caruso, Margit, Omega, Pallina as the pollen receiver and the S. phureja (GLWKS32508) as the pollen giver, were provided by the Julius Kuhn Institute of the Federal Republic of Gennany. The results showed that the conditions in which the mother plants were grown had significant effects on the production of hybrid fruits and seeds. The plants which were prevented from fonning potato tubers led to best results. Direct pollination of the potato stem carrying flowers growth in nutrient medium also led to better hybridization results than pollination of plants growth in nonnal conditions (without the restriction of producing tubers). The ploidy of the hybrid progenies was detennined by flow cytometry. The results indicated the most important role of the mother plants (the pollen receiver) in the reduction of the ploidy of the hybrid progenies. The progenies of the cultivar Omega were 100 percent diploid, while 100 percent of the hybrid progenies of the cultivars Caruso and Pallina with S. phureja were triploid (3x). The crosses between the cultivar Margit and Fasan with S. phureja provide the percentage of diploid progenies of 50 percent and 75 percent, respectively.Kết quả thu thập mẫu bệnh với những triệu chứng gây bệnh cháy lá trên sầu riêng tại tỉnh Tiền Giang từ tháng 3 đến tháng 12 năm 2019 thì 30 mẫu phân lập ra nấm Lasiodiplodia sp.
Phomopsis sp.
Colletotrichum sp. và Rhizoctonia sp. Trong đó nấm Lasiodiplodia sp.
Phomopsis sp. chiếm tỷ lệ cao 75 - 95% trong tất cả mẫu phân lập. Dựa trên đặc điểm hình thái như màu sắc của khuẩn lạc, khuẩn ty, hình dạng bào tử cũng như dùng sinh học phân tử (ITS) gồm điện di sản phẩm PCR cho sản phẩm khuếch đại vùng trình tự vùng ITS-rDNA
giải trình tự vùng ITS-rDNA và so sánh tương đồng bằng công cụ BLAST trên GeneBank, cho thấy hai mẫu nấm có trình tự vùng ITS tương đồng cao (98 - 100%) với loài Lasiodiplodia theobromae và Phomopsis durionis là tác nhân gây bệnh cháy lá sầu riêng ở Tiền Giang. Bệnh do loài Lasiodiplodia theobromae gây cháy ở đuôi lá màu trắng bạc trên lá già, trên vết bệnh có nhiều hạch nấm màu nâu đen trên vết bệnh cũ. Triệu chứng bệnh do loài Phomopsis durionis gây ra vết bệnh có những đốm bằng đầu kim, mỗi vết bệnh có quầng vàng xung quanh, vết bệnh có dạng oval, nặng có hình mắt cua có màu tro hay nâu dọc theo gân chính lan dần vào bên trong lá.

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