AIMS: To investigate the clinical features and characteristics of asymptomatic, acute and chronic MG infections in heterosexual males. METHODS: From January 2018 to September 2023, men diagnosed with urethral STIs using multiplex real-time PCR were enrolled into this cross-sectional study. Testing indications included males with urethritis, suspected STIs and asymptomatic screening. Based on clinical symptoms, participants were classified as acute, chronic or asymptomatic groups. An electronic-based survey was administered to assess demographic, behavioural and clinical information. Univariate and multivariate logistic regression were performed to assess for factors associated with the presence of symptoms. RESULT: Among the 2102 men who have sex with women and were tested for STI, 879 records met the eligibility criteria for further analysis
MG was detected in 14.6% of the 2102 cases. Most patients presented with acute urethral symptoms (75.6%). Among men infected with MG, mono-infection accounted for 52.8%, while CONCLUSION: Our study revealed a 14.6% prevalence of MG among individuals tested for STIs. Most patients presented with acute urethral symptoms, while chronic symptoms were associated with marriage, antibiotic use history and previous STIs. Routine MG screening of asymptomatic patients is not recommended, but improved access to diagnostics and further research is needed for clinical practice in low-resource settings.