So far fragrance of rice has mainly been determined by the traditional sensory method. This evaluation is fast but does not define precisely because it depends entirely on subjective assessment of the evaluator's olfaction. To overcome this shortcoming, the modern physico-chemical methods, SDE-GCFID and SDE-GCMS were employed. In these analytical processes, quantitative analysis of 2-AP content in pandan's leaf was performed to serve as basis for comparison with that in aromatic rice. By these methods, since 2005, 2-AP and some other aromatic compounds in aromatic rice have been identified and quantified in the Physicochemical Laboratory, Center for Biological and Chemical analysis and experiment, University of Agriculture and Forestry Ho Chi Minh city. In this laboratory from 2010, the content of 2 -AP in the old, mature and young leaves of Pandanus were accurately quantified and used as a standard for quantification of 2-AP in fragrant rice using SDE (or SPME) extraction method combined with GC-FID and GC-MS. The limit of detection (LOD) and limit of quantification (LOQ) of the method were also determined.