Phân lập và xác định trình tự hoàn chỉnh cdna mã hóa hemocyanin liên quan đến đáp ứng miễn dịch ở tôm sú (penaeus monodon) / Phân lập và xác định vi khuẩn từ vùng sinh thái bản địa có khả nàng đối kháng với Xanthomonas spp. gây bệnh đốm lá trên cây hoa hồng [Rosa spp.]

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Tác giả: Thị Thu Yến Hoàng, Thị Phương Oanh Kim, Uyển Thanh Lê, Đức Độ Nguyễn, Văn Hải Nông, Anh Tuấn Phạm, Lan Phương Tô, Đình Giỏi Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 660635 Chemical engineering and related technologies

Thông tin xuất bản: Tạp chí công nghệ sinh học, Nông nghiệp và Phát triển nông thôn, 20122021

Mô tả vật lý: 49-58, 29 - 35

Bộ sưu tập: Metadata

ID: 482288

Thêm vào giỏ

Hemocyanin is a large, copper-containing protein present in the hemolymph of both mollusks and arthropods. The traditional role of hemocyanin is the transport and storage of molecular oxygen. Today, it is known that hemocyanin is a multifunctional protein, especial function related to antivirus, antimicrobial and antifungal immune responses. A partial coding sequence of cDNA encoding hemocyanin consisted of 1470 nucleotides including stop codon and 3'-UTR is available from GenBank with accession number AF431737. In this study, the authors cloned and sequenced of the full length cDNA encoding syntenin from WSSS infected black tiger shrimp. Using 5'RACE technique, the authors determined 5'-end sequence including initial codon and 5'-UTR. Based on the 5'-end sequence and the GenBank sequence (AF431737), the complete hemocyanin cDNA was cloned and sequenced. The coding region of hemocyanin cDNA was 2052 bp in length, and was predicted to encode a 683 amino acid protein. The putative protein contains 3 domains and 6 conversed histidines to set structure and function. The result of nucleotide sequence alignment between the newly determined hemocyanin sequence and that of WSSV infected Penaeus monodon isolated in India showed that there are 33 different nucleotides in the two sequences. The nucleotide differences were found to result in 7 amino acid substitutions including 242H-D, 304N-D, 360V-I, 443K-L, 453Y-F, 472A-P and 576G-A. The results would be useful for further study of the function of hemocyanin and related proteins in the shrimp immune response.Từ 3 vùng sinh thái bản địa là Vườn Quốc gia Tràm Chim, Khu du lịch sinh thái Gáo Giông, Khu di tích lịch sử văn hóa xẻo Quýt thuộc tỉnh Đồng Tháp, 543 dòng vi khuẩn được phân lập và được khảo sát khả năng đối kháng đối với 3 dòng Xanthomonas spp. (XR9, XR13, XR18) gây bệnh đốm lá trên cây hoa hồng (Rosa spp.). Kết quả ghi nhận 17 dòng vi khuấn bản địa có khả năng đối kháng tích cực (D >
15 mm) đối với cả ba dòng gây bệnh. Trong đó, 3 dòng T265, G24, X61 đối kháng triển vọng với đường kính vùng ức chế cao nhất, đã được chọn xác định danh pháp. Qua kỹ thuật MALDI-TOF-MS, 2 dòng X61, G24 được xác định đều thuộc về chi Bacillus và dòng X61 được xác định đến loài là B. subtilis, trong khi chưa xác định được dòng T265. Hai dòng T265 và G24 tiếp tục dược xác định qua kỹ thuật giải trình tự gen 16S rRNA. Kết quả cho thấy dòng T265 có giá trị tương đồng cao nhất với Paenibacillus elgii MN160320 (98,50%), p. elgii HQ236085 (98,49%), trong khi dòng G24 tương đồng 99,49% với Bacillus subtilis MK859998 và EF530208.2. Dòng T265 được đề xuất danh pháp và accession number là Paenibacillus elgii MZ841643 và dòng G24 là Bacillus subtilis MZ841644. Các dòng vi khuẩn đối kháng này đã thể hiện tiềm năng kiểm soát vi khuẩn gây bệnh đốm lá trên cây hoa hồng ở Đồng Tháp.

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