Anther culturability of rice is significantly different between crosses rice varieties. A doubled haploid (DR) population was established via anther culture of some crossing from F1 on N6 medium, which had been shown particularly suitable for anther culture of indica hybrids. Among these factors, the most important one is the genotypic difference. The results found that different rice species, or crosses quite differently in response to anther culture. On the other hand, culture medium also strongly influences the anther culture response. The results that IR 64/OM 3536, OMCS 2000/ Jasmine M were which had high callus information rate. The results may be useful in the selection of parents with high response to anther culture for rice haploid breeding and in the establishment of permanent DH populations. There are 6 crosses F1 IR 64/0m 3536, IR 72/ OM 3536. OM 3536/ IR 73689-76, OM 3536/ IR 75997-159, OMCS 2000/ Jasmine M and Tequng/ OM 3536 give generation good for yield and also quality.