Strawberry is one of the most important fruit plant due to high nutritional value (particular in relatively high content vitamin C). Regeneration of strawberry through organogenesis has been studied, but its shoot multiplication was low
beside shoot has abnormalities such as vitrification and curliness on leaf. In order to overcome the weaknesses, in the present study, the authors reported a best regeneration protocol of strawberry through somatic embryogenesis. The leaf explants of strawberry were initially cultured on MS medium containing 1.0 mg/l TDZ and 2,4-D with different concentrations (0.1, 0.2 and 0.3 mg/l)
particularly in the experiment contains 1.0 mg/l TDZ and 0.2 mg/l 2,4-D combination which was very effective in inducing somatic embryos (the highest embryos number is 44.83 embryos). Somatic embryos were produced directly from the cut of leaf without intermediary callus phase after two months of culture. Regenerated plants transferred to soil,. showed that normal growth and morphology. This direct somatic embryogenesis from leaf in strawberry is a strong evidence of cell totipotency. The rapid somatic embryo induction protocol would be a useful tool for the propagation and regeneration.