The curent study was conducted to assess the oestrus synchronization on the total of 103 swamp buffalo cows. These animals' quality standards were good body condition scores and apparently healthy. The animals were raised in extensive production system on private farm. The buffalo cows were randomly assigned into three groups, the first group (35 buffalo cows) was treated (by Ovsynch program) with GnRH (fertagyl injection 2 ml) at zero day. At 7th day of implantation PGF2a Outalyse was injected 5 ml), then second GnRH (fertagyl was injected) at the 9th day, fiexd-time aticicial insemination, TAl at 16 h after second fertagyl treatment The second experimental group (35 buffalo cows) was treated by same program, but they injected with oestrogen at the 8th day instead of second fertagyl dose at 9th day, TAl at 24 h after oestrogen treatment. The third group (33 buffalo cows) CIDR was inserted at zero day with GnRH (injection of fertagyl2 ml), the CIDR was removed at the 7th day with PGF2a (injection of cloprosterol 2 ml). Oestrous detection and artificial insemination for natural heat buffalo cows to 11th day. All buffalo cows were artificially inseminated using frozen semen improted from India. It has been observed that all the three protocols buffalo cows tend to exhibit visible oestrous signs within 38.8 h after hormone administration. The results of the current study revealed that the averaged percentage of synchronized oestrous obtained was 73.79 percent and the pregnancy rate was 68.57 percent. The percentage of oestrous animals ranged from 65.71 percent (group 1), 75.76 percent (group 3) to 80 percent (P=0.417). Number of pregnant buffaloes after two Als on application of second protocol obtained 24 higher than of first 23 and of third 19 (P=0.621). It was found out that ovsych protocol with GnRH+PGF2a+ Oestrogen + TAl is a better treatment for oestrous synchronization (80 percent) and pregnancy (68.57 percent) in buffaloes. Oestrous synchronization with fixed time AI eliminates the practical problems of heat detection in buffaloes.