Tối ưu hóa nuôi cấy trong ống nghiệm và các giao thức chuyển đổi agrobacterium tumefaciens qua trung gian của thuốc lá by-2 tế bàoTối ưu hóa phối hợp bảo vệ rơ-le quá dòng có hướng sử dụng phương pháp tối ưu cá voi

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Tác giả: Minh Hải Đỗ, Phúc Khải Nguyễn

Ngôn ngữ: vie; Vie

Ký hiệu phân loại: 621 Applied physics

Thông tin xuất bản: Tạp chí khoa học, Tạp chí Khoa học và Công nghệ Đại học Đà Nẵng , 20122022

Mô tả vật lý: 128-137, 38-42

Bộ sưu tập: Metadata

ID: 482565

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In order to set up a system for production of recombinant proteins using suspension cell culture of Nicotiana tabacum cv. Bright Yellow 2 (BY-2), in vitro culture and genetic transformation protocols of such cells were optimised at the laboratory of Plant Cell Biotechnology, Institute of Biotechnology. The experiments indicated that the development rate of BY-2 cells depended on initial cell concentrations. The best culture condition was the formula F5 with 1:20 dilution at the starting point (2.5 mL of initial cells/50 mL culture medium) which reached the exponential phase after 5 days and had maximum biomass of 1.206 g/mL after 9 days. Agrobacterium tumefaciens-mediated transformation procedure of BY-2 cells was optimised by monitoring transient gusA gene expression. There is a positive correlation between the amount of BY-2 cells and the density of Agrobacterium tumefaciens in coculture medium. The transformation efficiency was the highest at formulars 0.6-1, 0.8-4 and 1.0-5 (bacteria OD-plant cell formular).Bài báo trình bày phương pháp tối ưu cá voi tính toán phối hợp bảo vệ tối ưu cho rơ-le bảo vệ có hướng trên lưới điện phân phối. Bài toán phối hợp bảo vệ rơ-le dùng các thuật toán tối ưu để tính toán phối hợp bảo vệ sao cho thời gian loại trừ sự cố trên lưới điện là nhỏ nhất. Trong nghiên cứu này, các tác giả sử dụng thuật toán tối ưu cá voi - một phương pháp tìm kiếm thông minh thế hệ mới và phần mềm MATLAB để tính toán, kiểm chứng các kết quả đạt được. Nghiên cứu được thực hiện trên lưới IEEE 3 nút, 8 nút và phát tuyến 473 Tân Xuân của lưới điện trung thế Tp. Hồ Chí Minh. So với các phương pháp tối ưu khác, các kết quả đạt được khi sử dụng thuật toán tối ưu cá voi được đánh giá tối ưu hơn và có khả năng áp dụng được vào lưới điện thực tế.

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