Objective: Investigate the proportion of thalassemia and other hemoglobinopathies in 12-18 age pupils from Muong ethnic group located in Kim Boi, Hoa Binh province. Materials and methods: 1224 pupils of secondary school in five villages: Vinh Dong, Hop Dong, Nam Thuong, Hop Kim, Kim Tien from Kim Boi district, Hoa Binh province. They were checked full blood count by automated hemotology analyser, fragility osmotic and haemoglobin analysis by HPLC. Results: The proportion of Hemoglobinopathies is very high, 20.12 percent. beta-thalassemia carrier (8.74 percent) , beta-thalassemia mild (0.25), beta-thalassemia major (0.16 percent), HbE (10.38 percent), E/beta-thal (0.25 percent), HbH (0.34 percent), HbD (0.16 percent).