Tocopherols with antioxidant properties are synthesized by photosynthetic organisms and play important roles in human and animal nutrition. In the major oilseed crops, gama-tocopherol, the biosynthetic precursor to alpha-tocopherol, is the predominant form found in the leaves. This suggests that the final step of the atocopherol biosynthetic pathway catalyzed by gama-Brassica oleracea tocopherol methyltransferase (gama-BoTMT). Transgenic Trichodenna reesei Rut-C30 strains, overexpressing the gama-BoTMT gene from the Brassica oleracea were generated by Agrobacterium tumefadens-mediated transformation. The presence of hph and gama-BoTMT gene in the transformants was confirmed by polymerase chain reaction (PCR) analysis. The expression of the gama-BoTMT gene of the transgenes was demonstrated by SDS-PAGE.