Tạo phôi soma từ mô sẹo ở giống cà phê vối (coffea canephora pierre) kháng tuyến trùng

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Tác giả: Thị Phương Loan Chu, Thị Tiếu Oanh Đinh, Ngọc Báu Lê, Thị Mai Nguyễn, Thị Thu Thủy Nguyễn, Thị Thúy Ngọc Nguyễn, Thị Hoàng Anh Trần, Thị Minh Huệ Trần, Văn Tân Trương

Ngôn ngữ: vie

Ký hiệu phân loại: 630 Agriculture and related technologies

Thông tin xuất bản: Công nghệ sinh học, 2012

Mô tả vật lý: 473-479

Bộ sưu tập: Metadata

ID: 483153

Nematode resistant coffee breeding is currently the top interest of Vietnam coffee sector. Results of collection and assessement on nematode resistance robusta found 10/24 is the most promissing material. However, the number of material for the study of next steps is limited. For nematode resistant material, vegetative propagation is a must. The propagation method of cutting gives low rate of multiplication. Tissue culture is the only technology that is able to provide a mass and identical material at the same time. Tissue culture can be implemented by micro-cutting or direct embryogenesis from leafsamples. However, this method brings a fairly low multiplication rate. In this study, the method of indirect embryogenesis was employed in order to get mass propagation for study and production of nematode resistant material. Three activities involved in the study include (1) the effect of regulators on callus formation, (2) the effect of liquid media on callus mass, and (3) the effect of culture media on embryogenesis from callus. Results show that the best medium for callus formation was MS plus BA 1 mg.l giving the highest percentage of samples generating callus (52.4 percent) after 16 weeks. Among different callus, the callus characteristics of light yellow and solid would be embryogenic callus. MS plus BA 1 mg.1 is also the best liquid medium for callus mass propagation. The best medium for callus embryogenesis was medium without regulator or with regulator of BA 0.25 mg.1.
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