Nghiên cứu nhân giống in vitro cây lan vân hài (paphiopedilum callosum) / Nghiên cứu nhân giống in vitro cây ngưu tất (Achyranthes bidentata Blume)

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Tác giả: Khắc Thịnh Đỗ, Tấn Nhựt Dương, Xuân Chiến Hoàng, Lê Thị Mận, Bá Nam Nguyễn, Phúc Huy Nguyễn, Nguyễn Thị Hồng Gấm, Trần Thị Thời, Thị Hương Trịnh, Quốc Luận Vũ, Thị Hiền Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 634.9 Forestry

Thông tin xuất bản: Công nghệ sinh học, Khoa học và Công nghệ Lâm nghiệp, 20122019

Mô tả vật lý: 497-505, 19-24

Bộ sưu tập: Metadata

ID: 483227

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In the present study, to 3-month-old shoots of Paphiopedilum callosum were used as source material for shoot regeneration. 2- and 3-month-old shoots cultured on SH medium supplemented with 1.0 mg/l TDZ, 0.3 mg/l NAA, 30 g/l sucrose and 9 g/l agar gave the best shoot regeneration (4.50 shoots/explant). Explants were efficiently surface sterilized by dipping in 0.1 percent fresh aqueous mercuric chloride (HgCl2) solution for 6 minutes. SH medium supplemented with 0.5 mg/l BA, 0.5 mg/l NAA, 30 g/l sucrose, 9 g/l agar, and 1 g/l activated charcoal was found to be suitable for the shoot growth and development. After 60 days of culture, utilizing the technique of wounding the shoot apex together with application of liquid SH medium in combination with cotton wool supplemented with TDZ at 0.4 - 0.6 mg/l and 30 g/l sucrose resulted in the highest number of shoots (4.50 shoots/explant). Root formation were successfully obtained when these shoots were transferred to SH medium supplemented with 1.0 mg/l NAA, 0.5 mg/l BA, 30 g/l sucrose, 9 g/l agar, and 1 g/l activated charcoal after 60 days of culture. All plantlets with well developed roots (100 percent) survived after the acclimatization period under greenhouse sonditions. This is the first time the technique of wounding the shoot apex was applied in P. callosum, and it showed to be a simple and effective micropropagation method for this recalcitrant species. Moreover, the technique should be considered for the breeding program of other species which belong to the genus Paphiopedilum.Nhân giống cây Ngưu tất (Achyranthes bidentata Blume) bằng kỹ thuật nuôi cấy mô đã được nghiên cứu thành công, kết quả cho thấy Hạt cây Ngưu tất được khử trùng bề mặt bằng cồn 70% (v/v) trong 1 phút, Javen 6% (v/v) trong 15 phút cho tỷ lệ mẫu sạch là 80,50% và tỷ lệ nảy mầm là 93,03%. Môi trường MS cơ bản bổ sung 0,1 mg/l NAA, 0,2 mg/l BAP, 0,1 mg/l Kinetin, 7 g/l agar và 20g/l sucrose là thích hợp để nhân nhanh, cho hệ số nhân nhanh chồi cao nhất đạt 10,7 chồi/mẫu và tỷ lệ chồi hữu hiệu là 91,20%. Môi trường MS bổ sung 0,3 mg/l NAA, 7 g/l agar và 20 g/l sucrose là thích hợp để ra rễ cho chồi in vitro, tỷ lệ chồi ra rễ đạt 100%, chiều dài rễ trung bình là 1,1 cm và số rễ trung bình trên chồi là 8,5 rễ/chồi. Cây con in vitro sinh trưởng tốt với thành phần ruột bầu là 75% đất, 25% cát , tỷ lệ sống cao nhất (83,33%), rễ phát triển tốt. Kết quả nghiên cứu cho thấy có thể ứng dụng phương pháp nuôi cấy in vitro vào nhân giống cây Ngưu tất, tạo ra số lượng cây giống lớn, chất lượng cao cung ứng cho nhu cầu trồng và phát triển loài cây dược liệu này

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