Quá trình hình thành phôi sinh dưỡng của cây địa lan "xanh chiểu" (cymbidium madrit "forest king")

 0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Tấn Nhựt Dương, Văn Cương Hoàng, Xuân Chiến Hoàng, Du Sanh Nguyễn, Thị Ngọc Lan Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 580 Plants

Thông tin xuất bản: Công nghệ sinh học, 2012

Mô tả vật lý: 507-517

Bộ sưu tập: Metadata

ID: 483230

In this study, embryogenic calli were induced from longitudinal thin cell layers of protocorm-like bodies of Cymbidium Madrit "Forest King", a hybrid orchid, on Murashige and Skoog medium (1962) supplemented with NAA alone or in combination with BA within 1 month. The medium containing the combination of 0.5 mg/l NAA and 0.1 mg/l SA in the light condittion was optimal for callus formation. Calli multiplied with 16.7 fold fresh mass increase when they were subcultured once a month on the Schenk and Hildebrandt medium (1972) supplemented with 0.2 g/l glutamine, 50 g/l sucrose, 2 g/l activated coal, 0.5 mg/l NAA, 0.1 mg/l BA and 8 g/l agar. Somatic embryos were formed on the same medium but without plant growth regulators. Histological observation showed that there was the formation of somatic embryo structure. Somatic embryos derived from calli were able to develop into normal plantlets with shoots and roots on MS medium without plant growth regulators after about 4 months culture. No abnormal morphological changes were found in Cymbidium seedlings with using propagation methods via embryogenesis.
Tạo bộ sưu tập với mã QR

THƯ VIỆN - TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP.HCM

ĐT: (028) 71010608 | Email: tt.thuvien@hutech.edu.vn

Copyright @2024 THƯ VIỆN HUTECH