Laccases are multi copper oxidoreductases that catalyze the oxidation of various substituted phenolic compounds. Laccases are widely distributed in higher plants and fungi. The ability of the laccases to act on a wide range of substrates has made them very important in many of the biotechnological applications. Fungi are now gained more and more attention because of their high potential for laccase synthesis. They were isolated from many different sources such as water, soil and wood. To estimate synthesis of laccase and degradation of polycyclic aromatic hydrocarbons and dye decolourization of fungi on different environments including forest, this study was conducted. In this study, FCP3 fungal strain from Cuc Phuong National Forest was screened for laccase sythesis with initial activity 11.7 UI on the Vis medium. FCP3 strain gave the highest laccase in the medium with pH 5.5
1 mM CuSO4 as an inducer, 10g/l glucose as a carbon source and 3 g/l mixture of KNO3 and NaNO3 as nitrogen sources. The degradation of anthracene and pyrene induced by FCP3 live cells were 33 and 41 percent, respectively and crude enzyme extracted from FCP3 were 41 percent and 54 percent, respectively (initial concentration of each PAH was 100 mg/l). Moreover, the crude enzyme extracted from FCP3 was able to decolour a variety of textile dyes: 92 percent NY3, 86 percent RBBR, 64 percent NY5, 60 percent NY1 and 6 percent NY7. Based on traditional method and determination of partial sequence of 18S rRNA gene, FCP3 strain was classified in genus Trichoderma and named Trichoderma. sp FCP3.