Background: Bleeding, bile duct injuries are not rare in laporoscopic cholecystectomy. Among the cause of that is the variation in cystic artery, cystic duct anatomy. The purpose study is to establish detailed and determine the rate of cystic artery, cystic duct in laparoscopic cholecystectomy. Meterial and methods is the prospective study on 69 laparoscopic cholecystectomies in Can Tho university of medicine and pharmacy from May 2012 to April 2013. Result Cystic duct: Cystic duct met commotl hepatic from the right and top duodenal to creat bile duct (94,3 percent). Cystic duct short met common hepatic to creat bile duct (4,3 percent). Low cystic duct merge with common hepatic duct, cystic duct and common hepatic duct (1,4 percent). Cystic artery Group cystic artery was found within hepatobiliary triangle 87 percent including 3 subgroups. Subgroup 1a: onlyone cystic artery was found within hepatobiliary triangle 69,6 percent, subgroup 1b: 2 or 3 branches of cystic artery 17,4 percent, subgroup 1c: cystic arteries arised from a right hepatic artery hump proximate to the gallbladder neck 0 percent. Group another blood supply was found outside hepatobiliary triangle in addition to the branches of cystic artery inside 7,2 percent including 2 subgroups. Subgroup 2a: the other branch of cystic artery courses outside the hepatobiliary triangle, inferio-Iateral to the cystic duct 2,9 percent. Subgroup 2b: the other branch of cystic artery was found in the liver bed 4,3 percent. Group there was no artery in the hepatobiliary triangle, only one cystic artery inferio-Iateral to the cystic duct was found 5,8 percent. Conclusions The anatomy of the cystic artery, cystic duct are of great change. Therefore, meticulous dissection during laparoscopic cholecystectomy must be obtained for a clear view of anatomical landmarks and the contents of hepatobiliary triangle to make low complications.