ĐÁNH GIÁ CHẤT LƯỢNG, HOẠT TÍNH KHÁNG OXY HÓA VÀ TÍNH AN TOÀN CỦA CAO CORDY-X TRÊN MÔ HÌNH NGUYÊN BÀO SỢI NGƯỜI VÀ ẤU TRÙNG TÔM NƯỚC MẶN Artemia franciscana

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Tác giả: Anh Duy Lê, Đăng Khoa Nguyễn, Minh Chánh Nguyễn, Thị Liên Thương Nguyễn, Thị Mai Trần

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học Đại học Công Thương (Tên cũ: Tạp chí Khoa học Công nghệ và Thực phẩm), 2024

Mô tả vật lý: tr.108

Bộ sưu tập: Metadata

ID: 483777

Thêm vào giỏ

Ganoderma lucidum and Cordyceps militaris are two major medicinal mushrooms with long-term usage in traditional and modern medicine. Along with the development of the research and cultivation of medicinal mushrooms, numerous commercial products, such as functional and supplemented foods, have been produced. Among them, the CORDY-X mushroom extract is the product that has the main ingredients of C. militaris and G. lucidum mushroom extracts. The Institute of Applied Technology, Thu Dau Mot University researched and transferred this product. In this study, we evaluated the product’s quality standards, quantified major bioactive compounds, adenosine, and cordycepin, by the UPLC method, determined the antioxidant activity against DPPH free radicals, and assessed cytotoxicity on human fibroblast cells and toxicity on Artemia franciscana shrimp. The results showed that the product meets quality standards. The adenosine and cordycepin contents were 0.95 mg/g and 0.26 mg/g, respectively. The product scavenged 27% of DPPH free radicals at a 250 µg/mL concentration. The product is not toxic to human fibroblasts or to Artemia franciscana at concentrations of 500 µg/mL and 1000 µg/mL. The findings clarify some pharmaceutical ingredients, antioxidant activity, and the safety level of pharmaceutical herbal products.Ganoderma lucidum và Cordyceps militaris là hai loại nấm dược liệu quý có nhiều ứng dụng trong y học cổ truyền và y học hiện đại. Cùng với sự phát triển của việc nghiên cứu và nuôi trồng nấm, nhiều sản phẩm thương mại như thực phẩm chức năng, thực phẩm bảo vệ sức khỏe từ nấm dược liệu đã được sản xuất. Trong đó, sản phẩm cao nấm CORDY-X có thành phần chính là cao nấm đông trùng hạ thảo và cao nấm linh chi, được nghiên cứu và chuyển giao bởi Viện Phát triển Ứng dụng, Trường Đại học Thủ Dầu Một. Trong nghiên cứu này, nhóm tác giả đã đánh giá chỉ tiêu chất lượng của sản phẩm, xác định hàm lượng nucleoside bằng phương pháp sắc ký lỏng hiệu năng cao, đánh giá hoạt tính kháng oxy hóa bằng phương pháp bắt gốc tự do DPPH (2,2-Diphenyl-1 picrylhydrazyl) và kiểm tra độ an toàn của sản phẩm trên mô hình tế bào và ấu trùng tôm, Artemia franciscana. Kết quả cho thấy sản phẩm đạt các chỉ tiêu chất lượng. Hàm lượng adenosine và cordycepin được xác định tương ứng là 0,95 mg/g và 0,26 mg/g. Hoạt tính bắt gốc tự do DPPH đạt khoảng 27% tại nồng độ 250 µg/mL. Ngoài ra, sản phẩm không có thể hiện độc tính trên nguyên bào sợi người và trên Artemia franciscana tại nồng độ lần lượt 500 µg/mL và 1000 µg /mL. Kết quả nghiên cứu góp phần làm rõ thêm thông tin về dược chất, hoạt tính và độ an toàn của sản phẩm cao nấm dược liệu.

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