The Xylaria nigripes fungus is one of the medicinal materials that was used in traditional medicine. Extracts from the mycelium and fruiting bodies of the X. nigripes showed protecting cells, preventing DNA damage, and reducing the programmed death cell rate. In this study, the fruiting bodies of the X. nigripes were collected to isolate, propagate in the liquid medium, and then cultured to induce the fruiting body formation in the rice medium. The results showed that the X. nigripes was successfully isolated on PDAY (Potato Dextrose Agar Yeast Extract) medium, and the mycelium and spore of the X. nigripes had unique characteristics of the species. The biomass of X. nigripes grew well in a liquid nutrient medium containing 20g glucose, 1g MgSO4.7H2O, 2g KH2PO4, and 5g peptone, reaching the best mass after 21 to 28 days. Using an initial cultured liquid of X. nigripes to culture on rice medium, fruiting bodies were formed and developed after 14 days of culture. This result could potentially be applied to the production of X. nigripes biomass via mycelium and fruit bodies for application in medicine.