The existance of chrommium in water at trace levels is mainly in the forms of Cr(III) and Cr(VI), in which Cr(VI) is toxic and difficult to do the treatment. To study the cumulative impact and risk of chromium in water and waste water to human health, the solid phase extraction process has been studied on XAD-7 resin to separate and enrich the existing forms of chromium. Factors affecting the yield such as pH, ligand amount, the amount of adsorbent, sample loading rate through the column, solvent volume and elution volume, sample volume, and the strange ions have been investigated. Optimum conditions have also been addressed. The process of chromium quantitative analysis is done by means of flame atomic absorption spectroscopy.