This study was conducted to evaluate the effect of Bovine Oviduct Epithelial Cells (BOECs) on the in vitro development of in vitro fertilized bovine embryos. BOECs were isolated by modified method of Walter (1995). They were in vitro cultured by modified method of Kim et al. (2011), the in vitro culture was carried out in Sanyo incubator at 37°C, 5 percent CO2, humidified air. BOECs were cultured in supplemented Dulbecco's Modified Eagle Medium (D'MEM). BOECs' monolayer was developed in modified method of Mc Gowan (2011). Bovine oocytes were matured by modified method of Duc et al. (2003). Selected sperms were prepared by Swim-up method (modified method of Parrish et al. (1986b)), that was performed in Brackett-Oliphant (BO) medium. After the different periods (20, 30, 40, 50, 60, 70, 80 minutes), sperm concentration was automatically determinated by using Spermacue machine. In vitro fertilization between the matured oocytes and selected sperms was conducted in Sanyo incubator at at 5 percent CO2, 39°C, humidified air (modified method of Duc et al. (2003)). In vitro fertilization was carried out in BO medium supplemented 1.0 uM Epinepherine, 10 uM Hypotaurine, 20 uM Penicillamine, 10 ug/ml Heparine. Bovine embryos were cultured in TC199 medium supplemented Fetal Bovine Sreum 10 percent, in the presence or absence of the BOECs. Results indicated that: 1/ After 60 minutes of Swim-up, the authors have got enough selected sperms' quantity for in vitro fertilization
2/ Proportion of embryo at 2-4 cell and 4-8 cell stage in medium were not significant differences (P0,05), for non-BOCEs and BOECs trials, that were 69,39 percent vs. 70,34 percent and 54,71 percent vs. 53,61 percent, respectively
3/ Proportion of embryos at morula and blastocyst stages were significant differences (P0,01), for non-BOCEs and BOECs trials, that were 17,20 percent vs. 30,34 percent, respectively. In conclusion, BOECs have affected positively the in vitro development of in vitro fertilized bovine embryos.