Cấu trúc tương tự thể giả hành trong nhân giống in vitro cây hoa lily lai (lilium spp)

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Tác giả: Tấn Nhựt Dương, Quốc Tuấn Phạm

Ngôn ngữ: vie

Ký hiệu phân loại: 660 Chemical engineering and related technologies

Thông tin xuất bản: Công nghệ sinh học, 2013

Mô tả vật lý: 249-253

Bộ sưu tập: Metadata

ID: 484100

In this study, the propagule of Lilium hybrids whose structure is similar to PLB of orchids were observed when bulb-scale slices (1 - 1.5 mm in thickness) were cultured on MS medium (Murashige, Skoog, 1962) supplemented with different concentrations of NAA and TDZ. Highest number of PLSs (23 PLSs per explant) were recorded on MS basal medium supplemented with 0.5 mg/l TDZ and 0.2 mg/l NAA. These PLSs could be maintained and proliferated at their early stage of development as which of Cymbidium. PLSs were cut vertically and cultured on different kinds of nutrient medium for proliferating or plantlet regenerating. About 12 plantlets were regenerated from 1 PLS cultured on MS basal medium supplemented with 1.0 g/l AC and 30 g/l sucrose, and about 598 (explant per bulb scale) x 23 (PLSs per explant) x 13 (plantlets per PLSs) plantlets were obtained from one bulb scale in one year via this protocol. In addition to propagation purpose, Lily PLS can be considered as a novel model for morphogenesis and transgenic studies.
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