NGHIÊN CỨU VAI TRÒ CỦA RAU HÚNG LŨI TRONG ỨC CHẾ SINH TRƯỞNG TẾ BÀO BGC-823=CYTOTOXIC EFFECT OF MENTHA CRISPA L. AGAINST HUMAN GASTRIC CANCER CELL LINES

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Tác giả: Văn Minh Lê, ĐạI HùNg Ngô, Minh KháNh TrầN, Thanh Sang Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học - Đại học Thủ Dầu Một, 2024

Mô tả vật lý: tr.45724

Bộ sưu tập: Metadata

ID: 484163

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Gastric cancer is presently ranked as the most prevalent type of cancer and stands among the top causes of cancer-related mortality globally. In this study, the cytotoxic activity of Mentha crispa extract against gastric cancer cells was investigated using an in vitro model. The extract was obtained through maceration with 98% ethanol. The cytotoxic ability of the extract was investigated using the MTT assay. The expression levels of intracellular signaling molecules were assessed using qRT-PCR. The result showed that Mentha crispa extract can decrease BGC-823 gastric cancer cell proliferation with an IC50 value of 130.5 ± 7.2µg/ml. Additionally, the inhibitory activity on the migration of gastric cancer cells in vitro by Mentha crispa extract was also determined at a concentration of 65µg/ml. Notably, cells treated with Mentha crispa extract at a concentration of 130µg/ml showed increased expression levels of apoptosis-related signaling molecules such as Caspase-3, -8, -9, and Bax. These results suggest that Mentha crispa extract has the potential to suppress gastric cancer cell proliferation through the activation of the apoptosis pathway, thereby inhibiting and eliminating the cells.Ung thư dạ dày trở nên phổ biến và gây ra tỷ lệ tử vong cao trên phạm vi toàn cầu. Nghiên cứu này tiến hành khảo sát ban đầu về khả năng tiêu diệt tế bào ung thư dạ dày của rau húng lũi thông qua mô hình in vitro. Cao chiết được thu nhận thông qua phương pháp ngấm kiệt bằng ethanol 98%. Khả năng tiêu diệt tế bào dạ dày của cao chiết được xác định thông qua phương pháp MTT. Mức độ biểu hiện của các phân tử tín hiệu nội bào được xác định thông qua kỹ thuật qRT-PCR. Kết quả khảo sát đã xác định khả năng tiêu diệt tế bào ung thư dạ dày BGC-823 của cao chiết rau húng lũi có giá trị IC50 đạt 130,5 ± 7,2µg/ml. Hơn nữa, khả năng ức chế sự di chuyển của tế bào ung thư dạ dày cũng được ghi nhận tại nồng độ 65µg/ml. Đáng chú ý, tế bào được xử lý với cao chiết rau húng lũi tại nồng độ 130µg/ml có thể làm tăng mức độ biểu hiện của các phân tử tín hiệu liên quan đến con đường apoptosis như Caspase-3,-8,-9, và Bax. Các kết quả trên cho thấy cao chiết rau húng lũi có tiềm năng tiêu diệt tế bào ung thư dạ dày thông qua hoạt hóa con đường apoptosis và gây ức chế di chuyển, tiêu diệt tế bào ung thư.

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