The authors conducted a cross-sectional study on 70 patients with CVC who had been at ED and ICU for more than 48 hours in the Bach Mai Hospital. The objective of this cross-sectional study was to study. Target (1) Identifying bacterial infection in patients suffering from central venous catheters (2) identify a number of factors related to infections acquired in patients with central venous catheters. Tools for data collection through protocol in patient's chart and medical records induding lab tests. The results showed the prevalence of nosocomial infection in post - Centre venous catheter patients was 15,7 percent, The risk factors for acquired CVC infection were the age group or = 60 and 60 with CVC (p 0,05), in patients with fever in CVC related infection and none CVC related group. (p 0,05). The Infection rate were significantly higher (p 0,05) in subclavical placement, three lumen catheter, three ways stopcocks and above 7 days CVC placement compared with carotid placement, one lumen catheter, less than three ways stopcock and less than 7 day CVC placement, corresponsively.