In this article, we investigate experimentally and numerically the performance of beams with web openings when subjected to two concentrated loads acting on the upper flange of the beam. Indicators such as: deflection between beams, stress at the edge of the hole, stress at the upper flange of the beam, etc. were measured during the experiment campaign. At the same time, numerical simulation of the experiment was performed using SAP2000 software. The numerical simulation analysis using SAP2000 software and experiment show very similar results. It can be concluded that numerical simulations using SAP2000 software can predict with high reliability and anticipate dangerous locations when designing with less cost than surveying by experiment.