Objectives: To define the kinds of bacteria in pediatric septic shock patients. Subjects and methods: a prospective descriptive study of 102 cases suffered from septic shock at the National Hospital of Pediatrics, during 3 years from 2006 to 2008, diagnostic criteria for septic shock as the international pediatric sepsis consensus conference 2002 (IPSCC 2002), identified the bacteria by blood culture before using antibiotics and culture body fluids, bacterial identification and do the antibiogram. Results: The rate of positive blood cultures was 14.7 percent, a positive blood culture is not related to outcome (survival/non-survivor). Of the 58 stains of bacteria isolated from blood and body fluids, rate of Gr (+) is 38 percent, Gr (-) is 59 percent, with 2 cases of Candida albicans infection. Image bacterias were: Klebsiella pneumoniae (22.4 percent), and others prospectively are (17.2 percent) of S. aureus, Pneumococcus, E.coli, H.influenza are 12,1 percent, 10.3 percent, 8.6 percent , 6.8 percent. On the antibiogram result, the Gr negative bacteria are resistant to neariy all of antibiotics, especially, the third generation cephalosporins, S. aureus are sensitive to almost antibiotics. Conclusions: The rate of positive blood cultures in children with septic shock is 14.7 percent, Gr negative bacteria have 59 percent, and 38 percent of Gr positive. The Gr negative bacteria are nearly resistant to all of antibiotics.