Khảo sát tỉ lệ hỗn hợp cao chiết từ lá Sen, thân rễ Nghệ và thân rễ Hoàng liên nhằm nâng cao hoạt tính ức chế enzym α-amylase và lipase tuyến tụy=Study on enhancing the inhibitory activity of pancreatic α-amylase and lipase enzymes of the mixture of Nelumbo nucifera leaves, Curcuma longa rhizomes and Coptis chinensis rhizomes

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Tác giả: Thị Thu Trang Lê, Đức Hoài Nam Nguyễn, Ngọc Tố Trinh Võ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2024

Mô tả vật lý: tr.64

Bộ sưu tập: Metadata

ID: 484256

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Các thuốc tổng hợp như orlistat và acarbose hỗ trợ giảm cân thông qua ức chế α-amylase và lipase nhưng gây tác dụng phụ. Khảo sát tỉ lệ hỗn hợp cao chiết từ lá Sen, thân rễ Nghệ và thân rễ Hoàng liên có hoạt tính ức chế α-amylase và lipase tuyến tụy. Dùng phần mềm Design-Expert 13 và mô hình D-Optimal để thiết kế thí nghiệm với ba biến độc lập là tỉ lệ (%) các cao chiết: lá Sen (X1), thân rễ Nghệ (X2) và thân rễ Hoàng liên (X3). Các giá trị X1, X2, X3 biến thiên từ 0 % đến 100 % và X1 + X2 + X3= 100 %. Hàm mục tiêu Y1 và Y2 lần lượt là giá trị IC50 α-amylase và lipase tuyến tụy. Kết quả tỉ lệ hỗn hợp cao chiết tối ưu (X1: 58,2 %, X2: 36,6 % và X3: 5,2 %) và dự đoán Y1 = 3 μg/mL và Y2 = 8 μg/mL với α = 0,05. Kết quả kiểm chứng bằng thực nghiệm không chênh lệch nhiều với giá trị dự đoán với IC50 của α-amylase và lipase của hỗn hợp là (3,043 ± 0,074) μg/mL và (8,183 ± 0,275) μg/mL, so với acarbose (2,645 ± 0,138) μg/mL và orlistat (1,630 ± 0,004) μg/mL. Hỗn hợp cao chiết được đề xuất có hoạt tính ức chế α-amylase và lipase tốt nhất so với các nghiệm thức khảo sát.Obesity is the cause of many diseases. Synthetic drugs such as orlistat and acarbose support weight loss through the inhibition of α-amylase and lipase but cause many side effects. Based on the results of recent studies on the weight loss effects of plants, the ratio of the mixture of extracts from Nelumbo nucifera leaves, Curcuma longa rhizomes, and Coptis chinensis rhizomes with the best inhibitory activity against pancreatic α-amylase and lipase was investigated by using Design-Expert 13 software and the D-Optimal model to design experiments with three independent variables: the percentage of the extracts: Nelumbo nucifera leaves (X1), Curcuma longa rhizomes (X2) and Coptis chinensis rhizomes (X3). The objective functions Y1 and Y2 are the IC50 values representing the inhibitory activity of α-amylase and pancreatic lipase enzymes, respectively. The results proposed the optimal extract mixture (X1: 58.2 %, X2: 36.6 %, and X3: 5.2 %) and predicted Y1 = 3 μg/mL and Y2 = 8 μg/mL with α = 0.05. Validation experiments showed that the IC50 inhibition of α - amylase and lipase enzymes of the optimal mixture was (3.043 ± 0.074) μg/mL and (8.183 ± 0.275) μg/mL, compared to acarbose (2.645 ± 0.138) μg/mL and orlistat (1.630 ±0.004) μg/mL. The proposed extract mixture had the best α-amylase and lipase inhibitory activity compared to the investigated mixtures.

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