Định lượng đồng thời các loại thuốc trong phác đồ điều trị nhiễm trùng Helicobacter pylori bằng phương pháp sắc ký lỏng hiệu năng cao=Quantification of different drugs in the treatment regimen for Helicobacter pylori infection using high performance liquid chromatography (HPLC) method

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Tác giả: Thanh Nhàn Mai, Thị Thu Thảo Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học và Công nghệ - Đại học Nguyễn Tất Thành, 2024

Mô tả vật lý: tr.72

Bộ sưu tập: Metadata

ID: 484262

Thêm vào giỏ

Phương pháp sắc ký lỏng hiệu năng cao được ứng dụng nhằm định lượng đồng thời sáu loại thuốc dùng trong điều trị nhiễm trùng Helicobacter pylori, gồm có famotidin, metronidazol, levofloxacin, rabeprazol natri, pantoprazol natri và lansoprazol. Việc phân tách thực hiện bằng cách dùng cột C18 (250 mm × 4,6 mm, 5 μm) và pha động gồm dung dịch đệm phosphate pH = 5,0 và acetonitril được bơm ở tốc độ dòng 1 mL·phút−1 bằng chương trình rửa giải gradient. Quá trình rửa giải gradient bắt đầu với tỷ lệ đệm phosphate:acetonitril là 9:1 (v
v), sau đó được thay đổi trong 25 phút để đạt tỷ lệ thể tích là 4:6 (v
v). Các chất phân tích được định lượng dựa trên việc đo diện tích peak của famotidin ở bước sóng 267 nm, metronidazol 320 nm, levofloxacin 294 nm, và rabeprazol natri, pantoprazol natri, lansoprazol 284 nm. Thẩm định quy trình phân tích theo hướng dẫn nội dung và phương pháp của ICH. Do đó, quy trình phân tích này có thể sử dụng trong việc kiểm tra hàm lượng của các hợp chất trên trong dược phẩm dạng thuốc viên.The research has applied the HPLC-PDA method to simultaneously quantify six types of drugs used in the treatment of Helicobacter pylori infection, including famotidine, metronidazole, levofloxacin, sodium rabeprazole, sodium pantoprazole, and lansoprazole. The separation was performed using a C18 reverse-phase column (250 mm × 4.6 mm, 5 μm) and a mobile phase consisting of a phosphate buffer solution at pH = 5.0 and acetonitrile, flow rate of 1 mL/min using a gradient elution program. The gradient elution started with a phosphate buffer:acetonitrile ratio of 9:1 (v/v), which was then changed to reach a ratio of 4:6 (v/v) over 25 minutes. The quantification of the analytes was based on the peak area measurements of famotidine at 267 nm, metronidazole at 320 nm, levofloxacin at 294 nm, and sodium rabeprazole, sodium pantoprazole, and lansoprazole at 284 nm. The validation of the analytical procedure, including system suitability, linearity, specificity, repeatability, and accuracy, met the requirements of the International Conference on Harmonization (ICH) guidelines. As a result, this analytical approach can be used to routinely monitor the quality of these chemicals in pharmaceutical dosage forms.

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