The native Bac Kan banana (Musa x paradisiaca var. "Bac Kan") (Musaceae) is amongst the important fruit crops that bring high income for growers in Bac Kan province. It has a high potential for large-cale production in the Northern region of Vietnam. However, the crop has been gradually degraded due to unsustainable production techniques, unfavourable impacts of weather conditions, diseases as well as the inadequate orientation and support of the local government. Under a component of propagation and intensive production for gene conservation and commercial development of native banana varieites for the region, this study was conducted during March - August 2013 to determine the most effective techniques at culture establishment stage of micropropagation for the variety. Four lab experiments were carried out to investigate influences of sucker sources from different mother plants' ages, positions of explant mateirals, and concentrations of two sterilizing chemicals, H2O2 and HgCl2, on alive and regeneration rates of shoots. As a result, shoot tips of suckers from one-year old mother plants were found to be the most effective materials among other. The use of the disinfectants did not increase regeneration rate, while high their concentrations seemed to have negative impacts.