Green tea (Camellia sinensis) belonging to the Theaceae family is widely planted and used in Asian, African, Latin American, and Oceanian countries. The plant is a nutrious source of polyphenols, catechins, amino acids, flavonoids, carotenoids, vitamins, and polysaccharides. Based on biochemical compounds in tea, tea has shown health benefits as anticancer, anti-oxidant, and antimicrobial activities, as well as its effectiveness in reducing body weight. In this study, there were 9 different bioactive compound groups detected in the green tea grown in Nghe An provinece, including alkaloids (22.01%), fatty acids (16.96%), triterpenoids (15.14%), phenols (9.50%), terpenes (9.43%), steroids (5.61%), ketones (4.79%), flavonoids (3.65%), and monoacylglycerols (1.96%), respectively. Particularly, the extreact of green tea leaves possessed ability to against Vibrio harveyi bacteria, which causes of serious diseases in aquatic animals such as lesions, blindness, gastroenteritis, myonecrosis, skin ulcers and tail rot in fish, and is the causative agent of luminous disease in fish, shrimp, crab... with high mortality rate. The antibacterial ring diameters were reported in the range of 10.82 mm and 16.52 mm under the extract concentrations of 200-500 ppm. This illustrates that the role and potential application of green tea extract in preventing and treatment bacterial diseases for aquatic animals, especially the luminous disease in shrimp. animals, especially the luminous disease in shrimp.