This study conducted a trial to mix three traditional medicinal herbs to make tea bags, such as Cordyceps militaris, Siraitia grosvenorii, and Chrysanthemum sinense. It investigated the chemical composition as well as the biological activity of the product. Cordyceps militaris, Siraitia grosvenorii, and Chrysanthemum sinense are precious medicinal species widely used in East Asian countries to make medicines and herbal b everages. However, research on the effects of tea bags with the above 3 ingredients has not yet been published in Vietnam. This study focuses on investigating the chemical composition, antioxidant, and anti-cancer activities of this tea bag to orient towards its application in food. The results of the study showed that the ratio of mixing Cordyceps militaris:Siraitia grosvenorii:Chrysanthemum sinense (w:w:w) of 2:1:1 obtained the highest sensory value and the basic chemical composition of the tea bag product was determined, including protein of 4%, the concentrations of polysaccharide of 23.4%, total polyphenol content of 56.34 mg GAE/g extract, adenosine of 0.183 mg/g extract, and cordycepin of 0.231 mg/g extract. An extract of the tea bag exhibited a free radical scavenging activity of DPPH with IC50 428.4 µg/mL. The concentration of the extract of the tea bag up to 1600 ppm induced cell death with 49.63% of A549 cells and 62.82% of MCF7 cells. In addition to showing the advantages of a tea bag product from Cordyceps militaris combined with Siraitia grosvenorii and Chrysanthemum sinense, this study has explored new research directions with potential applications in food for the first time.