Nghiên cứu về sự lưu hành của virus DIV1 gây bệnh trên tôm nuôi tại Việt Nam=Study on the circulation of DIV1 virus in farmed shrimp in Viet Nam

0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Xuân Khoa Âu, Văn Bắc Ngô, Đăng Hồng Ngọc Nguyễn, Thanh Loan Nguyễn, Thị Huyền Nguyễn, Thị Kim Oanh Nguyễn, Thị Thúy Mận Nguyễn, Đình Hoài Trương, Đăng Thắng Vũ, Thị Lan Hương Vũ

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2024

Mô tả vật lý: tr.20

Bộ sưu tập: Metadata

ID: 486334

Thêm vào giỏ

Nghiên cứu này đã được thực hiện để giám sát và đánh giá tình hình lưu hành của virus DIV1 trên tôm nuôi ở nước ta. Các mẫu: tôm nuôi (tôm thẻ chân trắng, tôm sú, tôm càng xanh), mẫu thức ăn tươi sống nuôi tôm bố mẹ, mẫu tôm giống kiểm dịch nhập khẩu, mẫu tôm được lưu giữ/bảo quản ở một số phòng thí nghiệm, mẫu tôm và giáp xác thu từ tự nhiên và mẫu tôm thu từ các ao nuôi nghi mắc bệnh đã được thu thập cho nghiên cứu này, số lượng mẫu thu đảm bảo tính đại diện về mặt không gian, mùa vụ, phương thức và quy mô nuôi khác nhau. Phương pháp nghiên cứu cắt ngang, lặp lại nhiều lần được sử dụng với mức đánh giá tình hình lưu hành của virus DIV1 trên tôm ở độ tin cậy là 95% và tỷ lệ lưu hành bệnh thiết kế là 4%. Tổng số mẫu đã thu thập được là 3.531 mẫu, trong đó có 2.067 mẫu tôm từ các hộ ương, nuôi
160 mẫu giun nhiều tơ
131 mẫu tôm và giáp xác tự nhiên
230 mẫu lưu/bảo quản từ các phòng thí nghiệm
360 mẫu tôm kiểm dịch
583 mẫu tôm nghi mắc bệnh. Kết quả xét nghiệm bằng kỹ thuật realtime và nested PCR cho thấy 100% số mẫu đều âm tính với DIV1. Như vậy trong phạm vi, đối tượng và thời gian nghiên cứu, chúng tôi chưa phát hiện thấy sự lưu hành của virus DIV1 ở Việt Nam.This study was conducted to detect and evaluate the circulation of DIV1 in the farmed shrimps in our country. The samples from shrimp farms (white leg shrimp, black tiger shrimp, giant freshwater shrimp), live feeds for broodstock, imported quarantine shrimp samples, conservated shrimp samples in the aquatic laboratories, wild shrimp and crustacean samples, and suspected diseased shrimp were collected for this study. The number of these collected samples were representative for locations, seasons, farming model and scales. A repeated cross-sectional study method was used with a 95% confidence level and 4% of design prevalence of the DIV1 infection in the farmed shrimps. The results of DIV1 analysis by real-time PCR and nested PCR techniques revealed that there were 3,531 samples collected, including 2,067 shrimp samples from rearing households, 160 polychaetes samples, 131 wild shrimp and crustacean samples, 230 conserved samples in the laboratories, 360 samples of quarantined shrimp, 583 disease suspected samples examined. As a result, 100% samples were negative with DIV1. Thus, within the study’s scope, subjects and time, we confirmed that the circulation of the DIV1 virus was not found in Viet Nam.

1. Đọc trực tuyến

1. Đọc trực tuyến

Tạo bộ sưu tập với mã QR