Phân lập và tuyển chọn chủng vi khuẩn Bacillus subtilis tiềm năng sử dụng sản xuất chế phẩm sinh học=Isolation and selection of potential Bacillus subtilis strain for producing probiotics

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Tác giả: Khả Hồng Đức Hồ, Minh Đức Lê, Thị Hoài Chúc Lê, Thị Hoa Nguyễn, Xuân Hoà Nguyễn, Thái Bình Phạm, Thị Hằng Phan, Vũ Hải Phan

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2024

Mô tả vật lý: tr.64

Bộ sưu tập: Metadata

ID: 486364

Thêm vào giỏ

Nghiên cứu được tiến hành tại khoa Chăn nuôi Thú y, Trường Đại học Nông Lâm, Đại học Huế nhằm phát hiện chủng Bacillus subtilis (B. subtilis) tiềm năng ứng dụng trong sản xuất chế phẩm vi sinh phục vụ chăn nuôi. Tổng số 27 khuẩn lạc thuần khiết có đặc điểm hình thái giống với Bacillus đã được phân lập. Kết quả giám định sinh hóa cho thấy B. subtilis (3/27 chủng), B. pumilis (4/2 chủng), B. lichenifomis (2/27 chủng), B. coagulans (3/27chủng), B. spharicus (3/27 chủng) và Bacillus sp. (12/27 chủng) đã được xác định. Vi khuẩn thuộc chi B. subtilis, B. pumilis và B. sphaericus có khả năng lên men đường lactose và glucose, không sinh hơi, tuy nhiên chỉ có B. subtilis có thêm khả năng phân giải tinh bột và protein. Trong ba chủng (B4, B17 và B23) được kiểm tra sinh hoá đều là B. subtilis, chúng tôi đã chọn chủng B23 để giải trình tự gen 16S ribosomal RNA, kết quả cho thấy chủng B. subtilis- B23 có trình tự gen tương đồng 100% với B. subtilis strain H1. Kết quả khảo sát đường cong sinh trưởng của Bacillus subtilis-B23 cho thấy sinh trưởng đạt số lượng cực đại sau 10 đến 14 giờ trong điều kiện nuôi cấy tĩnh với tỷ lệ tiếp giống 1%. Chủng B. subtilis-B23 có nhiều đặc tính sinh học quý, tiềm năng cho các nghiên cứu để chế tạo probiotics phục vụ chăn nuôi.The study was conducted at the Faculty of Animal Husbandry and Veterinary Medicine, Hue University of Agriculture and Forestry, Hue University to detect the potential strains of Bacillus subtilis (B. subtilis) for producing probiotics for animal husbandry. A total of 27 pure colonies having the morphological characteristics similar to Bacillus were isolated. The results of biochemical examination showed that there were B. subtilis (3/27 strains), B. pumilis (4/27 strains), B. lichenifomis (2/27 strains), B. coagulans (3/27 strains), B. spharicus (3/27 strains) and Bacillus sp. (12/27 strains) identified. Bacteria belonging to the B. subtilis, B. pumilis and B. sphaericus genus had the ability to ferment sugars without producing gas, but only B. subtilis had the ability to break down starch and proteins. Three strains (B4, B17 and B23) that were tested biochemically, they were all as B. subtilis, B23 strains was chosen for sequencing the 16S ribosomal RNA gene, as a result the similarity level of B23 and B. subtilis strain H1 was 100%. The surveyed result on the growth curve of B. subtilis - B23 showed that the growth reached the maximum number after 10 to 14 hrs. in the static culture conditions with the rate of seeding 1%. The B. subtilis-B23 strain has many potential valuable biological properties for research on creating probiotics for animal husbandry.

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