Sự có mặt của virus cúm gia cầm độc lực cao trên gia cầm sống bán tại một số chợ thuộc địa bàn Tỉnh Khánh Hòa=The presence of highly pathogenic avian influenza viruses in live poultry selling at some markets in Khanh Hoa province

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Tác giả: Thị Chính Lê, Thái Bình Nguyễn, Xuân Hòa Nguyễn, Hoàng Sơn Hưng Phạm, Nguyên Thảo Trần

Ngôn ngữ: vie

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Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2024

Mô tả vật lý: tr.1

Bộ sưu tập: Metadata

ID: 486381

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Nghiên cứu được tiến hành trên địa bàn tỉnh Khánh Hòa, 140 mẫu gộp dịch hầu-họng và phân của gà/vịt đã được thu thập tại các chợ bán gia cầm sống nhằm phát hiện sự có mặt của virus cúm A/H5N1, H5N6, H5N8 và H7N9. Đã sử dụng realtime quantitative reverve transcription PCR (RT-qPCR) để phát hiện và định type virus cúm. Kết quả nghiên cứu cho thấy số mẫu gia cầm dương tính với cúm A là 46/140 mẫu (chiếm tỷ lệ 32,86%), 3/140 mẫu dương tính với cúm A/H5N1 (chiếm tỷ lệ 2,14%) và tất cả mẫu còn lại âm tính với H5N6, H5N8 và H7N9. Tỷ lệ mẫu gia cầm dương tính với virus cúm A tại chợ Vĩnh Hải là cao nhất (22/35 mẫu
62,86%), trong đó có 3 mẫu dương tính với H5N1. Chợ Thành, Cam Hải và Sông Cạn đều có 8/35 (22,86%) mẫu gia cầm dương tính với cúm A và tất cả mẫu còn lại âm tính với A/H5 và A/H7. Virus cúm A xuất hiện ở tất cả các tháng thực hiện giám sát, tỷ lệ gia cầm nhiễm cúm cao nhất vào tháng 4 (42,86%) và thấp nhất vào tháng 3 (28,57%). Các tháng 3, 4 không phát hiện thấy virus cúm A/H5 và H7, trong khi đó lại phát hiện virus cúm A/H5N1 vào tháng 5 (2,14%). Kết quả nghiên cứu này là cơ sở cho tỉnh Khánh Hoà đưa ra các biện pháp phòng chống thích hợp hạn chế lây lan của virus cúm gia cầm.The study was conducted in Khanh Hoa province, there were 140 oropharyngeal swabs and fecal samples of chickens/ducks collected in the live-poultry markets to detect the presence of influenza A virus, subtype H5N1, H5N6, H5N8, and H7N9. Realtime quantitative reverse transcription - PCR (RT-qPCR) was used to detect the influenza viruses. The studied results showed that the infection rate of samples with influenza A virus was 46/140 samples (32.86%), while 3/140 samples (2.14%) were positive with A/H5N1, and all the rest samples were negative with H5N6, H5N8, and H7N9. The rate of positive samples with influenza A virus in the Vinh Hai market was the highest with 22/35 samples (62.86%), of which, 3 samples were positive with H5N1. There were 8/35 samples (22.86%) in Thanh, Cam Hai and Song Can markets positive with influenza A virus, and all the rest samples were negative with A/H5 and A/H7. Influenza A viruses were found in all studied months, the highest positive rate was in April (42.86%) and the lowest positive rate was in March (28.57%). In March and April, the influenza A/H5 and A/H7 viruses were not found, but H5N1 viruses were identified in May (2.14%). The results of this study would serve as a basis for Khanh Hoa province in marking appropriate prevention measures against the influenza A virus.

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